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PF060 TdT, Calf Thymus

TdT, Calf Thymus MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Terminal Deoxynucleotidyl Transferase

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PF060
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
PF060-.155ML Plastic ampoule .155 ml
Description
OverviewNative TdT from calf thymus. Tdt is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxy terminus of either double of single stranded DNA in a template-independent manner.
Catalogue NumberPF060
Brand Family Calbiochem®
SynonymsTerminal Deoxynucleotidyl Transferase
References
ReferencesChang, L.M.S. and F.J. Bollum. 1986. Crit. Rev. Biochem. 21, 27.
Deng, G. and R. Wu. 1983. Methods Enzymol. 100, 96.
Michelson, A.M. and S.H. Orkin. 1982. J. Biol. Chem. 257, 14773.
Roychoudhury, R. and R. Wu. 1980. Methods Enzymol. 65, 43.
Nelson, T. and D. Brutlag. 1979. Methods Enzymol. 68, 41.
Roychoudhury, R., et al. 1976. Nucleic Acids Res. 3, 101.
Bollum, F.J. 1974. In The Enzymes, 3rd edition (ed. P.D. Boyer), Vol. 10, 145. Academic Press, New York.
Product Information
Unit of DefinitionOne unit is the amount of enzyme required to transfer 1 nmol of dAMP from dATP to the 3ʹ-OH terminus of d(A)50 in 60 min at 37°C.
FormLiquid
Formulation775 Units in 50 mM potassium phosphate buffer, 1 mM B-mercaptoethanol, 50% glycerol, pH 7.2.
PreservativeNone
Quality LevelMQ100
Applications
Key Applications DNA Labeling
Application NotesDNA Labeling
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
PF060-.155ML 07790788054007

Documentation

TdT, Calf Thymus SDS

Title

Safety Data Sheet (SDS) 

TdT, Calf Thymus Certificates of Analysis

TitleLot Number
PF060

References

Reference overview
Chang, L.M.S. and F.J. Bollum. 1986. Crit. Rev. Biochem. 21, 27.
Deng, G. and R. Wu. 1983. Methods Enzymol. 100, 96.
Michelson, A.M. and S.H. Orkin. 1982. J. Biol. Chem. 257, 14773.
Roychoudhury, R. and R. Wu. 1980. Methods Enzymol. 65, 43.
Nelson, T. and D. Brutlag. 1979. Methods Enzymol. 68, 41.
Roychoudhury, R., et al. 1976. Nucleic Acids Res. 3, 101.
Bollum, F.J. 1974. In The Enzymes, 3rd edition (ed. P.D. Boyer), Vol. 10, 145. Academic Press, New York.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-September-2008 RFH
SynonymsTerminal Deoxynucleotidyl Transferase
ApplicationDNA Labeling
DescriptionNative TdT from calf thymus. Tdt is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxy terminus of either double of single stranded DNA in a template-independent manner. Although TdT preferentially adds deoxynucleotides to 3'-extensions, tailing onto 5' overhangs or blunt ended double strands of DNA can also be achieved. TdT can be used for labeling DNA fragments and vectors with homopolymer tails and/or with modified deoxynucleotides such as biotin-dNTPs, 32P-dNTPs, cordycepin-dNTPs, or ddNTP.
BackgroundThe Calbiochem® Terminal deoxynucleotidyl Transferase (TdT) is a DNA polymerase isolated from calf thymus. TdT catalyzes the addition of deoxynucleotides to the 3'-hydroxyl termini of either double or single stranded DNA molecules in a template independent manner. Although TdT preferentially adds deoxynucleotides to 3'-extensions, tailing onto 5' overhangs or blunt ended double strands of DNA can also be achieved. TdT can be used for labeling DNA fragments and vectors with homopolymer tails and/or with modified deoxynucleotides such as biotin-dNTPs, 32P-dNTPs, cordycepin-dNTPs, or ddNTP.
FormLiquid
Formulation775 Units in 50 mM potassium phosphate buffer, 1 mM B-mercaptoethanol, 50% glycerol, pH 7.2.
Unit definitionOne unit is the amount of enzyme required to transfer 1 nmol of dAMP from dATP to the 3ʹ-OH terminus of d(A)50 in 60 min at 37°C.
PreservativeNone
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesChang, L.M.S. and F.J. Bollum. 1986. Crit. Rev. Biochem. 21, 27.
Deng, G. and R. Wu. 1983. Methods Enzymol. 100, 96.
Michelson, A.M. and S.H. Orkin. 1982. J. Biol. Chem. 257, 14773.
Roychoudhury, R. and R. Wu. 1980. Methods Enzymol. 65, 43.
Nelson, T. and D. Brutlag. 1979. Methods Enzymol. 68, 41.
Roychoudhury, R., et al. 1976. Nucleic Acids Res. 3, 101.
Bollum, F.J. 1974. In The Enzymes, 3rd edition (ed. P.D. Boyer), Vol. 10, 145. Academic Press, New York.