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598226 Thiostrepton - CAS 1393-48-2 - Calbiochem

Thiostrepton, CAS 1393-48-2, is an antibiotic that inhibits protein synthesis by preventing binding of GTP to 50S ribosomal subunit.

Thiostrepton - CAS 1393-48-2 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: FOXM1 Inhibitor I

Primary Target: EF-G
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
1393-48-2C₇₂H₈₅N₁₉O₁₈S₅

Products

Catalog NumberPackaging Qty/Pack
598226-10GM Glass bottle 10 gm
598226-1GM Plastic ampoule 1 gm
Description
OverviewA thiazole-containing peptide antibiotic that inhibits protein synthesis by preventing binding of GTP to 50S ribosomal subunit. Inhibits the function of elongation factor G (EF-G) and the dissociation of EF-G from the ribosome. The thiostrepton-resistant gene is also commonly used as a selective marker for recombinant DNA/plasmid technologies.
Catalogue Number598226
Brand Family Calbiochem®
SynonymsFOXM1 Inhibitor I
References
ReferencesRodnina, M.V., et al. 1999. Proc. Natl. Acad. Sci USA 96, 9586.
Rosendahl, G. and Douthwaite, S. 1994. Nucleic Acids Res. 22, 357.
Saarma, U. and Remme, J. 1992. Nucleic Acids Res. 20, 3147.
Malina, H. and Robert-Gero, M. 1992. Appl. Environ. Microbiol. 58, 895.
Blanco, G., et al. 1992. Gene 112, 59.
Miller, S.P. and Bodley, J.W. 1991. Nucleic Acids Res. 19, 1657.
Ryan, P.C., et al. 1991. J. Mol. Biol. 221, 1257.
Kutay, U.R., et al. 1990. Biochim. Biophys. Acta 1050, 193.
Smokvina, T., et al. 1990. Gene 94, 53.
Garrett, R. 1983. Trends Biochem. Sci. 8, 189.
Bodley, J.W., et al. 1970. Biochem. Biophys. Res. Commun. 41, 1406.
Thompson, J. and Cundliffe, E. 1970. Biochimie 73, 1131.
Product Information
CAS number1393-48-2
FormOff-white powder
Hill FormulaC₇₂H₈₅N₁₉O₁₈S₅
Chemical formulaC₇₂H₈₅N₁₉O₁₈S₅
Quality LevelMQ100
Applications
Biological Information
Primary TargetEF-G
Purity≥98% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSXN6300100
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
598226-10GM 04055977189162
598226-1GM 07790788051914

Documentation

Thiostrepton - CAS 1393-48-2 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Thiostrepton - CAS 1393-48-2 - Calbiochem Certificates of Analysis

TitleLot Number
598226

References

Reference overview
Rodnina, M.V., et al. 1999. Proc. Natl. Acad. Sci USA 96, 9586.
Rosendahl, G. and Douthwaite, S. 1994. Nucleic Acids Res. 22, 357.
Saarma, U. and Remme, J. 1992. Nucleic Acids Res. 20, 3147.
Malina, H. and Robert-Gero, M. 1992. Appl. Environ. Microbiol. 58, 895.
Blanco, G., et al. 1992. Gene 112, 59.
Miller, S.P. and Bodley, J.W. 1991. Nucleic Acids Res. 19, 1657.
Ryan, P.C., et al. 1991. J. Mol. Biol. 221, 1257.
Kutay, U.R., et al. 1990. Biochim. Biophys. Acta 1050, 193.
Smokvina, T., et al. 1990. Gene 94, 53.
Garrett, R. 1983. Trends Biochem. Sci. 8, 189.
Bodley, J.W., et al. 1970. Biochem. Biophys. Res. Commun. 41, 1406.
Thompson, J. and Cundliffe, E. 1970. Biochimie 73, 1131.

Brochure

Title
Antibiotics Profiler

Citations

Title
  • Sarae L. Bausch, Ekaterina Poliakova and David E. Draper. (2005) Interactions of the N-terminal domain of ribosomal protein L11 with thiostrepton and rRNA. Journal of Biological Chemistry 280, 29956-29963.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision28-August-2014 JSW
    SynonymsFOXM1 Inhibitor I
    DescriptionA thiazole-containing polypeptide antibiotic isolated from Streptomyces azureus. Inhibits bacterial protein synthesis and ribosomal GTPase activity by binding noncovalently, but virtually irreversibly, to the 23S rRNA in the GTPase center of the 50S subunit. Thiostrepton binding directly prevents binding to the ribosome of elongation factor G (EF-G), which also binds specifically to the 23S rRNA. Thiostrepton has proved useful in elucidating the molecular mechanisms of translation, particularly of ribosomal translocation. It is also used for selection, in bacteria of Streptomyces sp., of vectors containing the tsr gene (S. azureus), which encode a 23S rRNA methylase whose action prevents thiostrepton from binding to ribosomes.
    FormOff-white powder
    CAS number1393-48-2
    RTECSXN6300100
    Chemical formulaC₇₂H₈₅N₁₉O₁₈S₅
    Purity≥98% by HPLC
    SolubilityDMSO (10 mg/ml) or Chloroform (10 mg/ml)
    Storage Protect from moisture
    Protect from light
    +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
    Toxicity Standard Handling
    Merck USA index14, 9364
    ReferencesRodnina, M.V., et al. 1999. Proc. Natl. Acad. Sci USA 96, 9586.
    Rosendahl, G. and Douthwaite, S. 1994. Nucleic Acids Res. 22, 357.
    Saarma, U. and Remme, J. 1992. Nucleic Acids Res. 20, 3147.
    Malina, H. and Robert-Gero, M. 1992. Appl. Environ. Microbiol. 58, 895.
    Blanco, G., et al. 1992. Gene 112, 59.
    Miller, S.P. and Bodley, J.W. 1991. Nucleic Acids Res. 19, 1657.
    Ryan, P.C., et al. 1991. J. Mol. Biol. 221, 1257.
    Kutay, U.R., et al. 1990. Biochim. Biophys. Acta 1050, 193.
    Smokvina, T., et al. 1990. Gene 94, 53.
    Garrett, R. 1983. Trends Biochem. Sci. 8, 189.
    Bodley, J.W., et al. 1970. Biochem. Biophys. Res. Commun. 41, 1406.
    Thompson, J. and Cundliffe, E. 1970. Biochimie 73, 1131.
    Citation
  • Sarae L. Bausch, Ekaterina Poliakova and David E. Draper. (2005) Interactions of the N-terminal domain of ribosomal protein L11 with thiostrepton and rRNA. Journal of Biological Chemistry 280, 29956-29963.
    		•