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14-763M Tie2 (R849W) Protein, active, 250 µg

Active, N-terminal 6His-tagged recombinant human Tie2 residues 771-end containing the R849W substitution. For use in Kinase Assays.

14-763M
250 µg  Also available in 10 μg size (cat#14-763) and in bulk (cat# 14-763-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-763M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionTie2 (R849W) Protein, active, 250 µg
OverviewN-terminal 6His-tagged recombinant human Tie2 residues 771-end containing the R849W substitution.
Background InformationThe R849W mutation has been reported in dominantly inherited venous malformations. Expression studies in insect and mammalian cells demonstrates that Tie2 R849W has constitutively elevated tyrosine kinase activity compared with wild type. (Morris P.N., et al., Biochem.Biophys.Res.Commun.(2006);346:335-338 and Vikkula M, et al., Cell(1996);87:1181-1190).
References
Product Information
Presentation50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Quality LevelMQ300
Applications
ApplicationActive, N-terminal 6His-tagged recombinant human Tie2 residues 771-end containing the R849W substitution. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cell.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • TEK
  • TIE2
Protein TargetTie2
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
Molecular Weight42kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-763) and in bulk (cat# 14-763-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
14-763M 04053252478925

Documentation

Related Products & Applications

Alternative Packsize

Catalog Number Description
14-763 Tie2 (R849W) Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases