Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

UC0E05 UCOE® Intermediate Vector Set

UCOE® Intermediate Vector Set MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
UC0E05
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberUC0E05
DescriptionUCOE® Intermediate Vector Set
Alternate Names
  • UCOE Mammalian Protein Expression Vectors
  • UCOE Mammalian Protein Expression Plasmids
  • Ubiquitous Chromatin Opening Element
  • UCOE05
Background InformationThe Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.

CET 1019 contains the 3 kb mouse Rps3 UCOE and the 1.5 kb fragment of human HNRPA2B1-CBX3 UCOE.
CET 1310 contains the 8 kb fragment of human HNRPA2B1-CBX3 UCOE.
CET 1316 contains the 4 kb fragment of human HNRPA2B1-CBX3 UCOE.

References
Antoniou, M., et al.. (2003) Transgenes Encompassing Dual Promoter CpG Islands from the Human TBP and HNRPA2B1 Loci are Resistant to Heterochromatin-mediated Silencing. Genomics, 82: 269-279.
Antoniou, M., et al. (2010) A Ubiquitous Chromatin Opening Element (UCOE) Confers Resistance to DNA Methylation-mediated Silencing of Lentiviral Vectors. Mol Ther, 18: 1640-1649.

THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.
References
Product Information
Components
  • 1) 10ug CET 1019 (3 kb Rps3 and 1.5kb HNRPA2B1-CBX3) Vector (CS221300)
  • 2) 10ug CET 1310 (8 kb HNRPA2B1-CBX3) Vector (CS221301)
  • 3) 10ug CET 1316 (4 kb HNRPA2B1-CBX3) Vector (CS221302)
Quality LevelMQ100
Applications
Application NotesUbiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression by removing epigenetic related gene silencing.
Biological Information
ConcentrationPlease refer to lot specific datasheet.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsPlasmids should be stored at -20C. Avoid repeated freeze-thaw events.
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
UC0E05 04054839077623