Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

23-029M UbcH5a Protein, active, 250 µg

Active, full length, recombinant human UbcH5a. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.

Key Applications: Enzyme Assays
View Products on Sigmaaldrich.com
23-029M
250 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Key ApplicationsSpeciesCascade Component
Enzyme AssaysHuman E2
Description
Catalogue Number23-029M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionUbcH5a Protein, active, 250 µg
OverviewFlocked swab:
Spray-on Nylon is the secret to flocked swab technology. The short, perpendicular fiber strands produced by the spray on method, creates an absorbent, thin-layer open structure that enhances both sample collection and release through the natural capillary movement of liquid along the fibers. Elution of the liquid and solid specimen sample is rapid and automatic because they are maintained close to the surface of the swab between the nylon strands, spontaneously draining when placed in liquid or onto solid media. Swab shafts are scored at a specific break-point for easy transfer of the tip into transportation media.

Universal Transport Medium (UTM):
UTM is provided with labeled capture-cap vials designed for transport of the clinical sample. The capture cap is designed to secure the shaft of the swab sample to the cap, eliminating the use of forceps to remove the swab in the laboratory.
Alternate Names
  • Ubiquitin-conjugating enzyme E2 D1
  • Ubiquitin-protein ligase D1
  • Ubiquitin-conjugating enzyme E2-17 kDa 1
  • UBC4/5 homolog
  • Stimulator of Fe transport (SFT)
  • UbcH5
  • UBE2D1
Background InformationUbiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or an Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate. The UbcH5 family (UbcH5a, UbcH5b and UbcH5c) have been shown to be the most active class of E2 enzymes in cell extracts and are associated with the regulation of a number of transcription factors, including p53 and c-Myc.
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, full length, recombinant human UbcH5a. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
Key Applications
  • Enzyme Assays
Biological Information
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Cascade ComponentE2
Entrez Gene Number
Purification MethodPurified using immobilized metal affinity chromatography.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size250 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
23-029M 04053252294457

Documentation

Related Products & Applications

Alternative Packsize

Catalog Number Description
23-029 UbcH5a Protein, active, 50 µg

Product Families

Ubiquitin Proteasome System: Enzymes and Substrates

Millipore has removed the complexity of Ubiquitination by providing functionally validated enzymes and substrates, making the pathway readily accessible for research and drug discovery.
Learn More >>

Categories

Life Science Research > Drug Discovery and Development > Ubiquitin and Proteasome Screening > Ubiquitin E2 Conjugating Enzymes
Life Science Research > Proteins and Enzymes > Ubiquitin E2 Conjugating Enzymes