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CBL202-K Sigma-AldrichVimentin Ms x Hu Mg

This Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin.

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Description
Catalogue Number	CBL202-K
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Vimentin Ms x Hu Mg
Overview	This product is intended for use by IVD manufacturers. Contact customer service for assistance: oem.reagents@emdmillipore.com
Alternate Names	Ant- Vimentin

References

Product Information
Format	Purified
Control	Positive in RD cells, glioma cells, fibroblasts (SV-80), and MDCK cell line
Presentation	Protein A Purified immunoglobulin presented as lyophilized material. Reconstitute with 1ml distilled water. Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Quality Level	MQ300

Applications
Application	This Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin.
Key Applications	ELISA Immunofluorescence Immunohistochemistry (Paraffin) Western Blotting
Application Notes	Western blot Immunohistochemistry: 1:2000; Frozen and paraffin-embedded tissue sections with a minimum of a one hour incubation (longer for paraffin); protease pretreatment is recommended for paraffin-embedded sections. Immunofluorescence ELISA Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Vimentin purified from bovine lens
Clone	VIM 3B4
Concentration	0.05 mg/mL
Host	Mouse
Specificity	Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This antibody is positive with cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. It can also be used to detect coexpression of vimentin in combination with other intermediate filament proteins. VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Polypeptide reacting: MW 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.
Isotype	IgG2a
Species Reactivity	Amphibian Bovine Canine Chicken Human Monkey
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_003380.2
Entrez Gene Summary	Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
Gene Symbol	VIM FLJ36605 Vimentin. vimentin
Purification Method	Protein A Purfied
UniProt Number	P08670
UniProt Summary	FUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. SIZE: 466 amino acids; 53652 Da SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein. TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines. PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family.
Molecular Weight	57 kDa

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Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at 2–8°C for up to 12 months from date of receipt.

Packaging Information
Material Size	Bulk packaging. Please inquire.

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Specifications

Global Trade Item Number
Catalog Number	GTIN
CBL202-K	04053252477430

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies

IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Secondary Bulk Antibodies > Anti-Human Antibodies & Conjugates > Mouse Anti-Human

IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Tumor Markers & Antigens

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