Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

GF175 WNT-1, Human Recombinant

WNT-1, Human Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture UniProt Number: P04628
View Products on Sigmaaldrich.com
GF175
10 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Key ApplicationsSourcePurity
CultE. coli.Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
Description
Catalogue NumberGF175
DescriptionWNT-1, Human Recombinant
Alternate Names
  • WNT1
  • Proto-oncogene Wnt-1
  • Proto-oncogene Int-1 homolog
Background InformationWnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, β-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.
References
Product Information
PresentationProduct is filtered through a 0.2 micron filter before lyophilization. .
Quality LevelMQ100
Applications
Key Applications
  • Stem Cell Culture
Biological Information
PurityProduct is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
SourceE. coli.
Cross ReactivityMouse
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceBioactivity assay: The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C for up to 6 months from date of receipt.
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
GF175 04053252691409

Documentation

WNT-1, Human Recombinant Certificates of Analysis

TitleLot Number
Recombinant Human WNT- 1 Recombinant Protein 3085049
Recombinant Human WNT-1 - 2146033 2146033
Recombinant Human WNT-1 - 2373513 2373513
Recombinant Human -2801960 2801960
Recombinant Human -2817266 2817266
Recombinant Human WNT- 1 - 2512052 2512052
Recombinant Human WNT- 1 - 3397991 3397991
Recombinant Human WNT- 1 - 3810155 3810155
Recombinant Human WNT- 1 - 3906397 3906397
Recombinant Human WNT- 1 - 4068651 4068651
Show more

Related Products & Applications

Product Families

Cytokines and Growth Factors

EMD Millipore offers a comprehensive range of highly purified bioactive cytokines and growth factors for use in human, mouse, and rat cell culture, stem cell differentiation and ELISA studies.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors