The concentration refers to the 10 µL loaded and is the concentration to be entered for the standard concentration in the analysis software. Refer to protocol page 7, Rat/Mouse Growth Hormone Standard Preparation, for the standard curve values.
EZRMGH
Rat/Mouse Growth Hormone ELISA Kit
measures and quantifies GH levels in 10 μL serum, plasma or cell culture supernatent
Synonym(s):
Growth hormone, Somatotrophin
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About This Item
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Product Name
Rat/Mouse Growth Hormone ELISA, This Rat/Mouse Growth Hormone ELISA is used to measure & quantify Growth Hormone levels in Metabolism & Endocrine & Cell Signaling research.
Quality Level
species reactivity
rat, mouse
packaging
kit of 1 × 96 wells
parameter
10 μL sample volume (4hr assay)
assay range
accuracy: 96-101%
linearity: 97-118%
sensitivity: 0.07 ng/mL
(10 μl sample size)
standard curve range: 0.07-50 ng/mL
technique(s)
ELISA: suitable
input
sample type serum
sample type cell culture supernatant
sample type plasma (K2 EDTA)
application(s)
research use
detection method
colorimetric (450nm/590nm)
shipped in
wet ice
storage temp.
2-8°C
General description
Application
Other Notes
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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With regard to what is loaded into each well (10 ul sample or standard plus 90 ul assay buffer) does the standard concentration (e.g 50 ng/ml) refer to the concentration of the 10 ul, or of the final 100 ul in the well? Thank you.
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