Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

70767 pET Expression System 23 - Novagen

pET vectors plus host strains with induction control

pET Expression System 23 - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70767
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
70767-3 Glass bottle 1 ea
Description
OverviewpET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock

Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid

These components are sufficient for up to 10 transformations in each host.

Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

pET Expression System 23
The pET Expression System 23 contains 10 µg each of the four versions of pET-23 (pET-23a–d(+)). pET-23(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It therefore lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His•Tag® sequence is available. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB051VM.




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number70767
Brand Family Novagen®
References
Product Information
•10 µg eachpET-23a-d(+) vector DNA
•0.2 mlHost bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
•0.2 mlInduction control clone, glycerol stock
Fusion tagT7•Tag, His•Tag
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
70767-3 04055977273199

Documentation

pET Expression System 23 - Novagen Certificates of Analysis

TitleLot Number
70767

Citations

Title
  • Faqiang Li, Ratnakar Vallabhaneni and Eleanore T. Wurtzel. (2008) PSY3, a new member of the phytoene synthase gene family conserved in the Poaceae and regulator of abiotic stress-induced root carotenogenesis. Plant Physiology 146, 1333-1345.
  • Christoph Edner, et al. (2007) Glucan, water dikinase activity stimulates breakdown of starch granules by plastidial beta-amylases. Plant Physiology 145, 17-28.
  • Rachel Pinto, et al. (2007) Sulfite reduction in Mycobacteria. Journal of Bacteriology 189, 6714-6722.
  • Ferdinand Steinboeck, et al. (2007) The nuclear actin-related protein of Saccharomyces cerevisiae, Arp4, directly Interacts with the histone acetyltransferase Esa1p. Journal of Biochemistry (Tokyo) 141, 661-668.
  • Feng Song, et al. (2006) Structure, function and mechanism of the phenylacetate pathway hot dog-fold thioesterase PAAI. Journal of Biological Chemistry 281, 11028-11038.
  • Tao Wu, et al. (2006) Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli. Procedings of the National Academy of Science 103, 11754-11759.
  • Antoine Attinger, et al. (2005) Molecular basis for the high affinity interaction between the thymic leukemia antigen and the CD8αα molecule. Journal of Immunology 174, 3501-3507.
  • Pedro Castanheira, et al. (2005) Activation, proteolytic processing and peptide specificity of recombinant cardosin A. Journal of Biological Chemistry 280, 13047-13054.
  • Laurent R Chiarelli, et al. (2005) Functional analysis of pyrimidine 5'-nucleotidase mutants causing nonspherocytic hemolytic anemia. Blood 105, 3340-3345.
  • James Flint, et al. (2005) Probing the mechanism of ligand recognition in family 29 carbohydrate-binding molecules. Journal of Biological Chemistry 280, 23718-23726.
  • Kiyotaka Fujita, et al. (2005) Identification and molecular cloning of a novel glycoside hydrolase family of core 1 type O-glycan-specific endo-α-N-acetylgalactosaminidase from Bifidobacterium longum. Journal of Biological Chemistry 280, 37415-37422.
  • Shingo Hanaoka, Aritaka Nagadoi and Yoshifumi Nishimura. (2005) Comparison between TRF2 and TRF1 of their telomeric DNA-bound structures and DNA-binding activities. Protein Science 14, 119-130.
  • Valerie Heurgue-Hamard, et al. (2005) The glutamine residue of the conserved GGQ motif in Saccharomyces cerevisiae release factor eRF1 Is methylated by the product of the YDR140w gene. Journal of Biological Chemistry 280, 2439-2445.
  • Middleton Boon Hinckley, et al. (2005) A Leptospira interrogans enzyme with similarity to yeast Ste14p that methylates the 1-phosphate group of lipid A. Journal of Biological Chemistry 280, 30214-30224.
  • Naomi Hosoya-Matsuda, et al. (2005) Anti-oxidative stress system in cyanobacteria: significance of type II peroxiredoxin and the role of 1-cys peroxiredoxin in Synechocystis sp. strain PCC 6803. Journal of Biological Chemistry 280, 840-846.
  • Shyh-Ing Jang, et al. (2005) Characterization of human epiplakin: RNAi-mediated epiplakin depletion leads to the disruption of keratin and vimentin IF networks. Journal of Cell Science 118, 781-793.
  • Andrew Karla, et al. (2005) The identification of residues that control signal peptidase cleavage fidelity and substrate specificity. Journal of Biological Chemistry 280, 6731-6741.
  • Laigeng Li, et al. (2005) Clarification of cinnamoyl co-enzyme a reductase catalysis in monolignol biosynthesis of aspen. Plant and Cell Physiology 46, 1073-1082.
  • Cishan Li, Michael E. Salvucci and Archie R. Portis, Jr.. (2005) Two residues of rubisco activase involved in recognition of the rubisco substrate. Journal of Biological Chemistry 280, 24864-24869.
  • Xu-Wen Liu, et al. (2005) Tissue inhibitor of metalloproteinase-1 protects human breast epithelial cells from extrinsic cell death: a potential oncogenic activity of tissue inhibitor of metalloproteinase-1. Cancer Research 65, 898-906.
  • Eugenia Mata-Greenwood, et al. (2005) Cyclic stretch increases VEGF expression in pulmonary arterial smooth muscle cells via TGF-{beta}1 and reactive oxygen species: a requirement for NAD(P)H oxidase. American Journal of Physiology: Lung 289, L288-L289.
  • Prachee Prakash, et al. (2005) Purified recombinant hypothetical protein coded by open reading frame Rv1885c of Mycobacterium tuberculosis exhibits a monofunctional AroQ class of periplasmic chorismate mutase activity. Journal of Biological Chemistry 280, 19641-19648.
  • Sadayappan Sakthivel, et al. (2005) In vivo and in vitro analysis of cardiac troponin I phosphorylation. Journal of Biological Chemistry 280, 703-714.
  • P. Szweda, R. Kotlowski and J. Kur. (2005) New effective sources of the Staphylococcus simulans lysostaphin. Journal of Biotechnology 117, 203-213.
  • Olga Vitavska, Hans Merzendorfer and Helmut Wieczorek. (2005) The V-ATPase Subunit C Binds to Polymeric F-actin as Well as to Monomeric G-actin and Induces Cross-linking of Actin Filaments. Journal of Biological Chemistry 280, 1070-1076.
  • Naohide Watanabe and Eric Lam. (2005) Two Arabidopsis metacaspases AtMCP1b and AtMCP2b are arginine/lysine-specific cysteine proteases and activate apoptosis-like cell death in yeast. Journal of Biological Chemistry 280, 14691-14699.
  • Bonney Wilkinson, Ruoyu Xiao and Hiram F. Gilbert. (2005) A structural disulfide of yeast protein disulfide isomerase destabilizes the active site disulfide of the N-terminal thioredoxin domain. Journal of Biological Chemistry 280, 11483-11487.
  • Liangwen Xiong, et al. (2005) Sites on calmodulin that interact with the C-terminal tail of Cav1.2 channel. Journal of Biological Chemistry 280, 7070-7079.
  • Hisashi Yagi, et al. (2005) Amyloid fibril formation of α-synuclein is accelerated by preformed amyloid seeds of other proteins: Implications for the mechanism of transmissible conformational diseases. Journal of Biological Chemistry 280, 38609-38616.
  • Roger B. Dodd, et al. (2004) Solution structure of the Kaposi's sarcoma-associated herpesvirus K3 N-terminal domain reveals a Novel E2-binding C4HC3-type RING. Journal of Biological Chemistry 279, 53840-53847.
  • Cynthia E. Gallagher, et al. (2004) Gene duplication in the carotenoid biosynthetic pathway preceded evolution of the grasses. Plant Physiology 135, 1776-1783.
  • Dan Gu, et al. (2004) Identification and characterization of the DNA-binding domain of the multifunctional PutA flavoenzyme. Journal of Biological Chemistry 279, 31171-31176.
  • Iwona K. Wower, Christian Zwieb and Jacek Wower. (2004) Contributions of pseudoknots and protein SmpB to the structure and function of tmRNA in trans-translation. Journal of Biological Chemistry 279, 54202-54209.
  • Assen Marintchev, Michael R. Gryk and Gregory P. Mullen. (2003) Site-directed mutagenesis analysis of the structural interaction of the single-strand-break repair protein, X-ray cross-complementing group1, with DNA polymerase β. Nucleic Acids Research 31, 580-588.
  • Cedric Orelle, et al. (2003) The conserved glutamate residue adjacent to the Walker-B motif is the catalytic base for ATP hydrolysis in the ATP-binding cassette transporter BmrA. Journal of Biological Chemistry 278, 47002-47008.
  • Yumi Maeda, et al. (2002) Novel 33-kilodalton lipoprotein from Mycobacterium leprae. Infection and Immunity 70, 4106-4111.
  • Diane C. Bassham, et al. (2000) AtVPS45 complex formation at the trans-golgi network. Molecular Biology of the Cell 11, 2251-2265.
  • Quinn L. Deveraux, et al. (1998) IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. European Molecular Biology Organization Journal 17, 2215-2223.
  • Pierre Martineau, Peter Jones and Greg Winter. (1998) Expression of an antibody fragment at high levels in the bacterial cytoplasm. Journal of Molecular Biology 280, 117-127.
  • K.K. Zimmerman, et al. (1998) High-level expression of rat farnesyl:protein transferase in Escherichia coli as a translationally coupled heterodimer. Protein Expression and Purification 14, (395- 402).
  • Stephen M. Black, et al. (1997) Ventilation and oxygenation induce endothelial nitric oxide synthase gene expression in the lungs of fetal lambs. Journal of Clinical Investigation 100, 1448-1458.
  • Thomas P. Shanley, et al. (1997) Requirement for C-X-C chemokines (macrophage inflammatory protein-2 and cytokine-induced neutrophil chemoattractant) in IgG immune complex-induced lung injury. Journal of Immunology 158, 3439-3448.
  • Anita Mistra-Press, et al. (1995) A novel mitogen-activated protein kinase phosphatase. Journal of Biological Chemistry 270, 14587-14596.
  • J.B. Dietrich, B. Lorber and D. Kern. (1991) Expression of mammalian tyrosine aminotransferase in Saccharomyces cerevisiae and Escherichia coli. European Journal of Biochemistry 201, 399-407.
    		•

    User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB051VM pET-23a-d(+) Vector Map