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70781 pET Expression System 30 - Novagen

pET vectors plus host strains with induction control

pET Expression System 30 - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70781
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
70781-3 Glass bottle 1 ea
Description
OverviewpET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock

Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid

These components are sufficient for up to 10 transformations in each host.

Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

pET Expression System 3
The pET Expression System 3 contains 10 µg each of the four versions of pET-30 (pET-30a–c(+)). The pET-30a–c(+) vectors carry an N-terminal His•Tag®/thrombin/S•Tag™/enterokinase configuration plus an optional C-terminal His•Tag sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB095VM.




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number70781
Brand Family Novagen®
References
Product Information
•10 µg eachpET-30a-c(+) vector DNA
•0.2 mlHost bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
•0.2 mlInduction control clone, glycerol stock
Fusion tagHis•Tag, S•Tag
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
70781-3 04055977273212

Documentation

pET Expression System 30 - Novagen Certificates of Analysis

TitleLot Number
70781

Citations

Title
  • Sarah A. Clock, et al. (2008) Outer membrane components of the Tad (tight adherence) secreton of Aggregatibacter actinomycetemcomitans. Journal of Bacteriology 190, 980-990.
  • Jeremy H. Herskowitz, et al. (2008) Systematic mutagenesis of the murine gammaherpesvirus 68 M2 protein identifies domains important for chronic infection. Journal of Virology 82, 3295-3310.
  • Anthony Argentaro, et al. (2007) Structural consequences of disease-causing mutations in the ATRX-DNMT3-DNMT3L (ADD) domain of the chromatin-associated protein ATRX. Procedings of the National Academy of Science 104, 11939-11944.
  • Srividya Balasubramanian, Sami R. Fam and Randy A. Hall. (2007) GABAB receptor association with the PDZ scaffold mupp1 alters receptor stability and function. Journal of Biological Chemistry 282, 4162-4171.
  • Meng Qi, Hyun-Sik Jun and Cecil W. Forsberg. (2007) Characterization and synergistic interactions of Fibrobacter succinogenes glycoside hydrolases. Applied and Enviornmental Microbiology 73, 6098-6105.
  • Satoshi Tasumi and Gerardo R. Vasta. (2007) A galectin of unique domain organization from hemocytes of the eastern oyster (Crassostrea virginica) is a receptor for the protistan parasite Perkinsus marinus. Journal of Immunology 179, 3086-3098.
  • Matthew R. Tubb, et al. (2007) Modulation of apolipoprotein A-IV lipid binding by an interaction between the N and C termini. Journal of Biological Chemistry 282, 28385-28394.
  • Kai-Hong Zhao, et al. (2007) Phycobilin:cystein-84 biliprotein lyase, a near-universal lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins. Procedings of the National Academy of Science 104, 14300-14305.
  • Chongguang Chen, et al. (2006) GEC1 interacts with the opioid receptor and enhances expression of the receptor. Journal of Biological Chemistry 281, 7983-7993.
  • Dmitri Nashchekin, et al. (2006) A novel ded1-like RNA helicase interacts with the Y-box protein ctYB-1 in nuclear mRNP particles and in polysomes. Journal of Biological Chemistry 281, 14263-14272.
  • Maryse Paquet, et al. (2006) The PDZ scaffold NHERF-2 interacts with mGluR5 and regulates receptor activity. Journal of Biological Chemistry 281, 29949-29961.
  • Gena D. Tribble, et al. (2006) A Porphyromonas gingivalis haloacid dehalogenase family phosphatase interacts with human phosphoproteins and is important for invasion. Procedings of the National Academy of Science 103, 11027-11032.
  • Yasuhiro Uchimura, et al. (2006) Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation. Journal of Biological Chemistry 281, 23180-23190.
  • Jiping Xiao, et al. (2006) Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis. Journal of Biological Chemistry 281, 15182-15193.
  • Kai-Hong Zhao, et al. (2006) Chromophore attachment to phycobiliprotein -subunits: phycocyanobilin:cysteine-84 phycobiliprotein lyase activity of Cpes-like protein from Anabaena SP. PCC7120. Journal of Biological Chemistry 281, 8573-8581.
  • Carmen Brenner, et al. (2005) Myc represses transcription through recruitment of DNA methyltransferase corepressor. European Molecular Biology Organization Journal 24, 336-346.
  • Min-Hsuan Chen, et al. (2005) Phospholipid scramblase 1 (PLSCR1) contains a non-classical nuclear localization signal with unique binding site in importin a. Journal of Biological Chemistry 280, 10599-10606.
  • Erika Crouch, et al. (2005) Ligand specificity of human surfactant protein D: expression of a mutant trimeris collection that shows enhanced interactions with influenza A virus. Journal of Biological Chemistry 280, 17046-17056.
  • Neelam Desai, et al. (2005) Two steps in Maf1 dependent repression of transcription by RNA Polymerase III. Journal of Biological Chemistry 280, 6455-6462.
  • Sanghamitra Dey, Gregory A. Grant and James C. Sacchettini. (2005) Crystal structure of mycobacterium tuberculosis D-3-phosphoglycerate dehydrogenase: extreme asymmetry in a tetramer of identical subunits. Journal of Biological Chemistry 280, 14892-14899.
  • Akari Fukuda, et al. (2005) Differential distribution of proteins expressed in companion cells in the sieve element-companion cell complex of rice plants. Plant and Cell Physiology 46, 1779-1786.
  • Jong-Min Han, et al. (2005) Structural and functional properties of V156K and A158E mutants of apolipoprotein A-I in the lipid-free and lipid-bound state. Journal of Lipid Research 46, 589-596.
  • Patricia L. Herman, et al. (2005) A three component dicamba O-demethylase from Pseudomonas maltophilia, strain DI-6: Gene isolation, characterization, and heterologous expression. Journal of Biological Chemistry 280, 24759-24767.
  • Bruce J. Herron, et al. (2005) A mutation in NFκB interacting protein 1 results in cardiomyopathy and abnormal skin development in wa3 mice. Human Molecular Genetics 14, 667-677.
  • Antonia H. Holway, Crystal Hung and W. Matthew Michael. (2005) Systematic, RNAi-mediated identification of mus-101 modifier genes in Caenorhabditis elegans. Genetics 169, 1451-1460.
  • J. K. Jang, T. Rahman and K. S. McKim. (2005) The kinesinlike protein subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. 16, 4684-4694.
  • Aki Jarvinen, et al. (2005) Metabolic stability of alpha -methylated polyamine derivatives and their use as substitutes for the natural polyamines. Journal of Biological Chemistry 280, 6595-6601.
  • Christopher S Kearn, et al. (2005) Concurrent stimulation of cannabinoid CB1 and dopamine D2 receptors enhances heterodimer formation: a mechanism for receptor crosstalk? Molecular Pharmacology 67, 1697-1704.
  • Torsten Kessler, et al. (2005) Inhibition of tumor growth by RGD peptide-directed delivery of truncated tissue factor to the tumor vasculature. Clinical Cancer Research 11, 6317-6324.
  • Alexei Kharitonenkov, et al. (2005) FGF-21 as a novel metabolic regulator. Journal of Clinical Investigation 115, 1627-1635.
  • Jui-Ming Lin, Analyne Schroeder and Ravi Allada. (2005) In vivo circadian function of casein kinase 2 phosphorylation sites in Drosophila PERIOD. Journal of Neuroscience 25, 11175-11183.
  • Margarita L. Malakhova, et al. (2005) Point mutational analysis of the liganding site in human glycolipid transfer protein: functionally of the complex. Journal of Biological Chemistry 280, 26312-26320.
  • Victoria L. Marsh, Sew Yeu Peak-Chew and Stephen D. Bell. (2005) Sir2 and the acetyltransferase, Pat, regulate the archael chromatin protein, Alba. Journal of Biological Chemistry 280, 21122-21128.
  • Satoru Moritoh, et al. (2005) RNAi-mediated silencing of OsGEN-L (OsGEN-like), a new member of the RAD2/XPG nuclease family, causes male sterility by defect of microspore development in rice. Plant and Cell Physiology 46, 699-715.
  • Catherine Paradis-Bleau, Francois Sanschagrin and Roger C. Levesque. (2005) Peptide inhibitors of the essential cell division protein FtsA. Protein Engineering Design and Selection 18, 85-91.
  • Kevin Pearson, et al. (2005) Specific sequences in the N-and C-termini of apolipoprotein A-IV modulate its conformation and lipid association. Journal of Biological Chemistry 280, 38576-38582.
  • Daniel Picard, C. Cheng Kao and Katalin A. Hudak. (2005) Pokeweed antiviral protein inhibits brome mosaic virus replication in plant cells. Journal of Biological Chemistry 280, 20069-20075.
  • Alexander J. Ruthenburg, et al. (2005) A superhelical spiral in the Escherichia coli DNA gyrase A C-terminal domain imparts unidirectional supercoiling bias. Journal of Biological Chemistry 280, 26177-26184.
  • Francois Sanschagrin and Roger C. Levesque. (2005) A specific peptide inhibitor of the class B metallo-{beta}-lactamase L-1 from Stenotrophomonas maltophilia identified using phage display. Journal of Antimicrobial Chemotherapy 55, 252-255.
  • Masako Shimada, et al. (2005) The receptor for parathyroid hormone and parathyroid hormone-related peptide is hydrolyzed and its signaling properties are altered by directly binding the calpain small subunit. Endocrinology 146, 2336-2344.
  • David Stuckler, et al. (2005)

    User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB095 pET-30a-c(+) Vector Map