Water for pH Measurement
Application Overview
Water for pH measurement and buffer preparation
The measurement of pH is one of the most widely performed tests in laboratories and industries. This is because many physical properties, chemical and biological process are dependent on pH. For example, the following are all influenced by pH:- Solubility of many chemicals
- Bio-availablity of drug moelcules
- Rates of chemical reactions
- Physiological chemistry of living organisms
The most accurate way of measuring pH is by using a pH meter. It is based on the difference of potential between two electrodes, one of them being a reference electrode. An approximate measure of pH may be obtained by using a pH indicator, a substance that changes color around a particular pH value. Universal indicators may also be used. They consist of a mixture of indicators such that there is a continuous color change from about pH 2 to pH 10. They also come in paper form, the universal indicator paper, which is a simple paper that has been impregnated with universal indicator.
A buffer is a solution that maintains its pH after the addition of a small amount of an acid or a base. Buffers are widely used in laboratories since pH influences the rate of many chemical and enzymatic reactions. Buffers and solutions used in pH measurements are usually prepared with high-purity water in order to minimize the risk of contamination from water impurities.
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