Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
If you have chosen panel analytes and then choose a premix or single plex kit, you will lose that customization.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Ultrapure water is used for many steps of blotting: sample, buffer, gels and rinsing solutions preparation. Prior to blotting itself, either for Southern or Northern blotting, the sample preparation implies a particular care of water quality used to protect raw material (respectively DNA or RNA).
The major sample preparation steps are the isolation by extraction, amplification and a pretreatment to denature RNA to prevent formation of base-paired secondary structure.
Water Quality Parameters
Ions Charged molecules can interfere during migration electrophoresis steps by changing the global charge of the solutions (buffer, sample…) and then either accelerating or delaying the migration.
The concentration of ions is also important during the hybridization step. In order to carry on the hybridization in optimum conditions, specific concentrations should be respected. Starting with water virtually free of ions (resistivity 18.2 MΩ•cm @ 25°C) ensures the preparation of the buffers at the right ionic strength and pH.
Organics Organic molecules can create interferences in the hybridization process. Those molecules that are negatively charged can behave just as the DNA and RNA and bind non-specifically in place of the DNA or RNA. Positively charged molecules can actually bind to the DNA or RNA and the oligonucleotides, retarding or interfering with the hybridization process. Consequently, low TOC value (< 10 ppb or µg/L) is recommended to ensure good water quality in terms of organic content.
Nucleases Nucleases degrade the NA samples. These enzymes are efficiently removed using ultrafiltration. The ultrafilter is installed at the outlet of the purification unit, just before water is drawn from the system and used. Please refer to the section on nuclease-free water.
Bacteria Bacteria liberate nucleases that degrade Nucleic Acids samples. They contain DNA, which can interfere with DNA to be checked. Finally, they release ions and organics that can have a negative effect on blotting steps Water purified with an Ultrafilter (BioPak) has bacteria levels < 0.1 cfu/mL.
In summary, considering the overall processes associated with the blotting steps, water should be nuclease-free and bacteria-free, have a high resistivity and a low TOC. All this can be obtained using an ultrafiltration device at the point-of-use (the outlet) of a water purification unit delivering high purity water. Please refer to the following sections for more information: Nuclease-free water, gel electrophoresis, PCR and DNA Sequencing.