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14-555M Alk Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant human ALK, residues 1058-end, for use in Kinase Assays.

Alk Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-555M
250 µg  Also available in 10 μg size (cat#14-555) and in bulk (cat# 14-555-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-555M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAlk Protein, active, 250 µg
      OverviewN-terminal His6-tagged, recombinant human ALK, residues 1058-end
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal His6-tagged, recombinant human ALK, residues 1058-end, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed by baculovirus in Sf21 cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.
      Gene Symbol
      • ALK
      • CD246
      • TFG/ALK
      Protein TargetALK
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyTK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UM73 # Orphan receptor with a tyrosine-protein kinase activity. Appears to play an important role in the normal development and function of the nervous system. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif.
      SIZE: 1620 amino acids; 176428 Da
      SUBUNIT: Homodimer. When bound to ligand.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
      PTM: N-glycosylated.
      DISEASE: SwissProt: Q9UM73 # A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas. & A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A. & A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
      SIMILARITY: SwissProt: Q9UM73 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 1 LDL-receptor class A domain. & Contains 2 MAM domains. & Contains 1 protein kinase domain.
      Molecular Weight63.8kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of IGF-1Rtide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C from date of shipment
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-555) and in bulk (cat# 14-555-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      14-555M 04053252378669

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-555 Alk Protein, active, 10 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases