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MABS2005-25UG Anti-2A peptide Antibody, clone 3H4

Anti-2A peptide, clone 3H4 Antibody, Cat. No. MABS2005-25UG, validated for use in Western Blotting, Immunofluorescence and Immunoprecipitation.

Anti-2A peptide Antibody, clone 3H4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function UniProt Number: N/A
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MABS2005-25UG
25 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, IF, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS2005-25UG
      DescriptionAnti-2A peptide Antibody, clone 3H4
      Background Information2A peptides and 2A-like peptide sequences (known as cis-acting hydrolase elements or CHYSEL) are a superior alternative to internal ribosomal entry sites (IRES) for coordinating the expression of multiple gene products from a single recombinant construct. The 2A sequences are relatively short peptides of about 20 amino acids (depending on the virus of origin) containing the consensus motif Asp-Val/Ile-Glu-X-Asn-Pro-Gly-Pro. 2A peptides allow multiple proteins to be encoded as polyproteins, which then dissociate into component proteins upon translation. The 2A sequence impairs normal peptide bond formation via a mechanism called ribosomal skipping, which results in effective, non-enzymatic generation of distinct peptide products from a single multicistronic construct. 2A peptides are used by several families of viruses, the best known foot-and-mouth disease virus of the Picornaviridae family, for producing multiple polypeptides. Anti-2A peptide, clone 3H4 recognizes 2A sequence derived from foot and mouth piconavirus (VKQTLNFDLLKLAGDVESNPG*P) with cleavage site between G and P. (Ref.: Trichas, G, et al. (2008). BMC Biol. 6: 40).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-2A peptide, clone 3H4 Antibody, Cat. No. MABS2005-25UG, validated for use in Western Blotting, Immunofluorescence and Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A representative lot detected 2A peptide in HEK293 SpCas9-P2A-Puro and NIH3T3 SpCas9-T2A-Puro cell lysates (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
      Biological Information
      ImmunogenKLH-conjuated linear peptide corresponding to sequence CGDVEENPG (free C-term) derived from Thosea asigna virus.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3H4 detects 2A peptide in murine cells.
      IsotypeIgG1
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH3T3 expressing SpCas9-T2A-Puro cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS2005-25UG 04054839421044