Millipore Sigma Vibrant Logo

MABE1829-25UG Anti-Acetyl β-Catenin (Lys49) Antibody, clone mAb-K49-Ac

Anti-Acetyl -Catenin (Lys49), clone mAb-K49-Ac, Cat. No. MABE1829, is a rat monoclonal antibody that targets Catenin -1 acetylated on Lysine 49 and is tested in Dot Blot, Immunoprecipitation, and Western Blotting.

MABE1829-25UG
25 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1829-25UG
      DescriptionAnti-Acetyl β-Catenin (Lys49) Antibody, clone mAb-K49-Ac
      Alternate Names
      • Catenin beta-1
      Background InformationCatenin beta-1 (UniProt: P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, OK/SW-cl.35, PRO2286) gene (Gene ID: 1499) in human. Catenin beta-1 is a key downstream component of the canonical Wnt signaling pathway that participates in intercellular adhesion and Wnt-mediated transcriptional activation. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK-3 beta that promotes phosphorylation on N-terminal serine and threonine residues that leads to its subsequent degradation by the proteasome. In the presence of Wnt ligand, it is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, which leads to activation of Wnt responsive genes. O-glycosylation at serine 23 is reported to decreases its nuclear localization and transcriptional activity. Wnt/beta-catenin signaling is shown to be essential for embryonic stem cell (ESC) pluripotency and differentiation. In ESC beta-catenin is trimethylated on lysine 49 by Ezh2 methyltransferase and acetylated by CBP. The trimethylated form represses neuronal differentiation genes Sox1 and Sox3. The K49 acetylated form acts as a transcriptional co-activator of the key mesodermal differentiation gene t-brachyury (t-bra). K49A mutant form of beta-catenin is shown cause defects in ESC differentiation. Mutations in CTNNB1 gene are shown to cause colorectal cancers, medulloblastoma, and ovarian cancers. (Ref.: Hoffmeyer, K., et al. (2017). Cell Reports 18(12); 2815-2824; Levy, L., et al. (2004). Mol. Cell Biol. 24(8); 3404-3414).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Acetyl -Catenin (Lys49), clone mAb-K49-Ac, Cat. No. MABE1829, is a rat monoclonal antibody that targets Catenin -1 acetylated on Lysine 49 and is tested in Dot Blot, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Dot Blot
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Acetyl a-Catenin (Lys49) in Western Blotting applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

      Dot Blot Analysis: A representative lot detected Acetyl -Catenin (Lys49) in Dot Blot applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Acetyl -Catenin (Lys49) in Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
      Biological Information
      ImmunogenBSA-conjugated linear peptide corresponding to 19 amino acids surrounding acetylated lysine 49 from human -catenin.
      ClonemAb-K49-Ac
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone mAb-K49-Ac is a rat monoclonal antibody that detects -Catenin acetylated on lysine 49.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Canine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CTNNB1
      • CTNNB
      • OK/SW-cl.35
      • PRO2286
      Modifications
      • Acetylation
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~90 kDa observed; 85.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SW 480 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Acetylated -Catenin (Lys49) in SW 480 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE1829-25UG 04061841440697

      Documentation

      Anti-Acetyl β-Catenin (Lys49) Antibody, clone mAb-K49-Ac SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Acetyl β-Catenin (Lys49) Antibody, clone mAb-K49-Ac Certificates of Analysis

      TitleLot Number
      Anti-Acetyl β-Catenin (Lys49), clone mAb-K49-Ac - Q3416339 Q3416339

      References

      Reference overviewPub Med ID
      Trimethylation and Acetylation of β-Catenin at Lysine 49 Represent Key Elements in ESC Pluripotency.
      Hoffmeyer, K; Junghans, D; Kanzler, B; Kemler, R
      Cell Rep  18  2815-2824  2017

      Show Abstract       		28329675 28329675