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MABS501 Anti-Anoctamin-5 Antibody, clone 5F7

This Anti-Anoctamin-5 Antibody, clone 5F7 is validated for use in Western Blotting for the detection of Anoctamin-5.

Anti-Anoctamin-5 Antibody, clone 5F7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: ANO5, GDD1, TMEM16E UniProt Number: Q75V66
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MABS501
200 µL  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWBMUnpurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS501
      DescriptionAnti-Anoctamin-5 Antibody, clone 5F7
      Alternate Names
      • Anoctamin-5
      • Gnathodiaphyseal dysplasia 1 protein
      • Transmembrane protein 16E
      Background InformationAnoctamin-5 (UniProt Q75V66; also known as Gnathodiaphyseal dysplasia 1 protein, Integral membrane protein GDD1, Transmembrane protein 16E) is encoded by the ANO5 (also known as GDD1, LGMD2L, TMEM16E) gene (Gene ID 203859) in human. The human Anoctamins (ANO) compose a family of at least ten proteins, all exhibiting eight transmembrane domains and a DUF590 domain of unknown function. The ANO1, ANO2, ANO6, ANO8, and ANO9 are characterized to code for the elusive calcium-activated chloride channels (CaCCs). While the physiological function of anoctamin-5 is not yet characterized, dominant mutations in ANO5 have been found in the rare skeletal disorder gnathodiaphyseal dysplasia. In addition, recessive mutations in ANO5 are found to cause proximal muscular dystrophy (LGMD2L) and distal Miyoshi myopathy (MMD3).
      References
      Product Information
      FormatUnpurified
      PresentationMouse monoclonal IgMκ in buffer containing supernatant without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Anoctamin-5 Antibody, clone 5F7 is validated for use in Western Blotting for the detection of Anoctamin-5.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:100 dilution from a representative lot detected Anoctamin-5 in 10 µg of mouse leg muscle tissue lysate.
      Biological Information
      ImmunogenLinear peptide corresponding to mouse Anoctamin-5 near the C-terminus.
      EpitopeNear C-terminus
      Clone5F7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgMκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteMouse, Human. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ANO5
      • GDD1
      • TMEM16E
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~37 kDa observed. ~37 kDa observed. Although the full-length protein has a calculated molecular weight of 107.2 kDa (Uniprot Q75V66), only a ~37 kDa band corresponding to a short spliced variant can be detected by Western blotting when using muscle tissue lysates. A band corresponding to the full-size protein and a 55 kDa band can be detected in addition to the 37 kDa band when using lysates prepared from human embryonal rhabdomyosarcoma (RMS) RD cells, C2C12 murine myoblasts, and fibroblasts.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse skeletal muscle tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Anoctamin-5 in 10 µg of mouse skeletal muscle tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS501 04055977175462