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MABT1318-25UG Anti-BCL-6 Antibody, clone GI191E/A8

MABT1318-25UG
25 μg  
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      Overview

      Description
      Catalogue NumberMABT1318-25UG
      DescriptionAnti-BCL-6 Antibody, clone GI191E/A8
      Alternate Names
      • B-cell lymphoma 6 protein
      • B-cell lymphoma 5 protein
      • BCL-5
      • Protein LAZ-3
      • Zinc finger and BTB domain-containing protein 27
      • Zinc finger protein 51
      Background InformationB-cell lymphoma 6 protein (UniProt: P41182; also known as BCL-6, B-cell lymphoma 5 protein, BCL-5, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51) is encoded by the BCL6 (also known as BCL5, LAZ3, ZBTB27, ZNF51) gene (Gene ID: 604) in human. BCL-6 is a homodimeric protein with six C2H2 type zinc finger domains that is expressed in germinal center T- and B-cells and in primary immature dendritic cells. It acts as a transcriptional repressor mainly required for germinal cell (GC) formation. In GC B-cells, it represses genes that function in differentiation, inflammation, apoptosis and cell cycle control. It allows GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunoglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. BCL-6 is also required for the establishment and maintenance of immunological memory for both T- and B-cells. Its levels are down-regulated during maturation of dendritic cells by selective stimuli such as bacterial lipopolysaccharides, CD40LG, and zymosan. BCL-6 levels are also shown to decline upon genotoxic stress in a dose- and time-dependent manner. Chromosomal aberrations involving BCL-6 are known to cause B-cell non-Hodgkin lymphomas.
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-BCL-6, clone GI191E/A8, Cat. No. MABT1318, is a mouse monoclonal antibody that detects B-cell lymphoma 6 (BCL-6) protein and has been tested for use in ELISA, Immunohistochemistry (Paraffin), Western Blotting.
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesEnzyme Immunoassay (ELISA) Analysis: A representative lot detected BCL-6 in ELISA applications (Garcia, J.F., et. al. (2006). J Histochem. Cytyochem. 54(1):31-8).

      Western Blotting Analysis: A representative lot detected BCL-6 in Western Blotting applications (Garcia, J.F., et. al. (2006). J Histochem. Cytyochem. 54(1):31-8).

      Immunohistochemistry (Paraffin) Analysis: A representative lot detected BCL-6 in Immunohistochemistry applications (Garcia, J.F., et. al. (2006). J Histochem. Cytyochem. 54(1):31-8; Nan-Cha, S.H., et. al. (2009). Mod Pathol. 22(8):1006-15).
      Biological Information
      ImmunogenpCDNA3‐BCL6 plasmid vector (full length sequence of human BCL-6).
      CloneGI191E/A8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone GI191E/A8 specifically detects BCL-6 in Human, Mouse, and Rat tissues.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteMouse, Human, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BCL6
      • BCL5
      • LAZ3
      • ZBTB27
      • ZNF51
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight78.85 kDa calculated.
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human tonsil tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:2,000 dilution of this antibody detected BCL-6 in human tonsil tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Global Trade Item Number
      Catalog Number GTIN
      MABT1318-25UG 04054839961748