Anti-BLAST-2 (CD23), clone EBVCS3

Clone EBVCS3 is a mouse monoclonal antibody that detects Low affinity immunoglobulin epsilon Fc receptor (CD23; BLAST-2).

Activity Assay Flow Cytometry Immunofluorescence

Anti-BLAST-2 (CD23), clone EBVCS3, Cat. No. MABF2318, is a mouse monoclonal antibody that detects Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) and is tested for use in Activity Assay, Flow Cytometry, and Immunofluorescence.

Flow Cytometry Analysis: A representative lot detected BLAST-2 (CD23) in Flow Cytometry applications (Stohl, W., et. al. (1990). Cell Immunol. 130(2):257-70).

Activity Assay Analysis: A representative lot detected BLAST-2 (CD23) in Activity Assay applications (Schattner, E.J., et. al. (1996). Blood. 88(4):1375-82)

Immunofluorescence Analysis: A representative lot detected BLAST-2 (CD23) in Immunofluorescence applications (Kintner, C., et. al. (1981). Nature. 294(5840):458-60).

EBV-transformed human cells.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Purified mouse monoclonal antibody IgM in PBS without preservatives.

Low affinity immunoglobulin epsilon Fc receptor (UniProt: P06734; also known as BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, CD23) is encoded by the FCER2 (also known as CD23A, CLEC4J, FCE2, IGEBF) gene (Gene ID: 2208) in human. Epstein-Barr virus (EBV) is reported to transform human B lymphocytes and is a causative agent for Burkitt s lymphoma and infectious mononucleosis. EBV induces the expression of one or more antigens (e.g., CD23) on the surface of adult human B-lymphocytes soon after infection. CD23 is a homotrimeric, single-pass type II membrane protein that serves as a low-affinity receptor for Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21 and plays an essential role in the regulation of IgE production and in the differentiation of B-cells. CD23 can also exist as a soluble excreted form, sCD23 (aa 150-321), which is produced by ADAM10-mediated ectoderm shedding. CD23 has a cytoplasmic domain (aa 1-21), a transmembrane domain (aa 22-47), and an extracellular domain (aa 48-321). Clone EBVCS3 can specifically detect antigens that are present on the surface of EBV-transformed cells. These antigens are also recognized by autochthonous cytotoxic T cells specific for EBV-transformed cells. (Ref.: Kintner, C., and Sugden, B. (1981). Nature. 294(3); 458-460).

Evaluated by Flow Cytometry in Raji B cells.

Flow Cytometry Analysis: 2 µg of this antibody detected BLAST-2 (CD23) in Raji cells.

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