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MABF3068-100UG Anti-CD163 Antibody, clone MAC2-158

This mouse monoclonal Anti-CD163, clone MAC2-158, Cat. No. MABF3068 is tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting, Flow Cytometry, Immunocytochemistry, for the detection of CD163.

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MABF3068-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3068-100UG
      DescriptionAnti-CD163 Antibody, clone MAC2-158
      Alternate Names
      • Scavenger receptor cysteine-rich type 1 protein M130
      • Hemoglobin scavenger receptor
      Background InformationScavenger receptor cysteine-rich type 1 protein M130 (UniProt: Q86VB7; also known as Hemoglobin scavenger receptor, CD163) is encoded by the CD163 (also known as M130) gene (Gene ID: 9332) in human. CD163 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to produce the mature form that contains a long extracellular domain (aa 42-1050), a transmembrane domain (aa 1051-1071), and a cytoplasmic domain (aa 1072-1156). The extracellular domain contains nine scavenger receptor cysteine-rich (SRCR) scavenger receptor class B domains of about 100 amino acids each. SRCR domain 3 mediates calcium-sensitive interaction with Hemoglobin/Haptoglobin (Hb/Hp) complexes. CD163 is expressed in monocytes and mature macrophages with high expression observed in bone marrow macrophages, Kupffer cells, lung macrophages, and red pulp macrophages. Its levels are up regulated by anti-inflammatory mediators and suppressed by pro-inflammatory mediators like lipopolysaccharides (LPS) and TNF . It serves as an acute phase-regulated receptor involved in clearance and endocytosis of Hb/Hp complexes by macrophages and protects tissues from free hemoglobin-mediated oxidative damage. The binding of the Hb/Hp complex to CD163 macrophages leads to a fast degradation of the complex. CD163 also plays a role in the uptake and recycling of iron, via endocytosis of Hb/Hp complex and subsequent breakdown of heme. A soluble form of CF163 (sCD163) has also been described that is produced by proteolytic shedding and can be induced by LPS, phorbol ester, and Fc region of immunoglobulin . This soluble form plays an anti-inflammatory role and is considered a diagnostic parameter for monitoring macrophage activation in inflammatory conditions. Its expression is markedly induced by anti- inflammatory mediators, such as glucocorticoids and interleukin 10. Clone MAC2-158 binds to SRCR domain 1 of CD163 and recognizes over 80% of circulating CD14-expressing monocytes. (Ref.: Zhi, Y., et al. (2017). Mol. Med. Rep. 15(5); 2931-2939; Maniecki, MB., et al. (2011). Immunology 216(8); 882-890; Morganelli, PM., and Guyre, PM. (1988). J. Immunol. 140(7); 2296-2304).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationThis mouse monoclonal Anti-CD163, clone MAC2-158, Cat. No. MABF3068 is tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting, Flow Cytometry, Immunocytochemistry, for the detection of CD163.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD163 in human liver tissue sections.

      Flow Cytometry Analysis: A representative lot detected CD163 in Flow Cytometry applications Maniecki, M.B., et al. (2011). Immunobiology. 216(8): 882-90; Krijgsman, D., et al. (2020). Int J Mol Sci. 21(16): 5925).

      Immunocytochemistry Analysis: A representative lot detected CD163 in Immunocytochemistry applications (Maniecki, M.B., et al. (2011). Immunobiology. 216(8): 882-90).

      Flow Cytometry Analysis: A representative lot detected CD163 in human peripheral blood mononuclear cells (PBMC) (Data courtesy of Jane Collins, Research Laboratory Manager of Dr. Paul M. Guyre s Lab, Department of Microbiology and Immunology, Geisel School of Medicine, Dartmouth College, NH, USA).

      Western Blotting Analysis: A representative lot detected CD163 in Western Blotting applications (Maniecki, M.B., et al. (2011). Immunobiology. 216(8): 882-90).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated CD163 in Immunoprecipitation applications (Morganelli, P.M., et al. (1988). J Immunol. 140(7): 2296-304).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenInterferon and Dexamethasone treated human monocytes.
      EpitopeN-terminal
      CloneMAC2-158
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone MAC2-158 is a mouse monoclonal antibody that detects human CD163. It targets an epitope within the SRCR1 domain from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CD163
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight125.45 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human thymus tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected CD163 in human thymus tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABF3068-100UG 04065268364475