Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
If you have chosen panel analytes and then choose a premix or single plex kit, you will lose that customization.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Please see lot specific datasheet for product formulation. We recommend that each laboratory determine an optimum working titre for use in its particular application.
This Anti-CD28 Antibody, clone 15E8 is validated for use in FC, FUNC for the detection of CD28.
Key Applications
Flow Cytometry
Affects Function
Application Notes
To study the role of CD28 in T cell proliferation. The antibody will induce the proliferation of resting T lymphocytes.
This antibody will act in cell stimulation studies where it has been suggested that measuring T cell function in HIV infected asymptomatic patients may be an early indicator of AIDS, independent of CD4 + cell counts
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Human T lymphocytes
Clone
15E8
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody reacts with the 44 kDa antigen found on a sub-population of T cells and activated B cells. These CD28+ CD8 cells are now known to be cytotoxic T cell precursors. The antibody does not react with B cells, granulocytes or monocytes. The ligand for CD28 is CD80 (B7) which is found on activated B cells suggesting a role for CD28 in T and B cell interaction
FUNCTION: SwissProt: P10747 # Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. SIZE: 220 amino acids; 25066 Da SUBUNIT: Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed in T-cells and plasma cells, but not in less mature B-cells. SIMILARITY: SwissProt: P10747 ## Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
