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ABS1627 Anti-CTGF Antibody, N-Term Antibody

This Anti-CTGF Antibody, N-Term Antibody is validated for use in Western Blotting, Neutralizing for the detection of CTGF.

Anti-CTGF Antibody, N-Term Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: CTGF, CCN2, HCS24, IGFBP8 UniProt Number: P29279
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ABS1627
100 μg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, NEUTGtAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1627
      DescriptionAnti-CTGF Antibody, N-Term Antibody
      Alternate Names
      • Connective tissue growth factor
      • CCN family member 2
      • Hypertrophic chondrocyte-specific protein 24
      • IBP-8
      • IGF-binding protein 8
      • IGFBP-8
      • Insulin-like growth factor-binding protein 8
      Background InformationConnective tissue growth factor (UniProt P29279; also known as CCN family member 2, Hypertrophic chondrocyte-specific protein 24, IBP-8, IGF-binding protein 8, IGFBP-8, Insulin-like growth factor-binding protein 8) is encoded by the CTGF (also known as CCN2, HCS24, IGFBP8) gene (Gene ID 1490) in human. CTFG is one the three founding members of the CCN (Ctgf, Cyr61, and Nov) family of proteins involved in a wide array of cellular activities, including migration, proliferation, extracellular matrix synthesis, adhesion, survival, differentiation, and apoptosis. CTGF acts an autocrine mediator of transforming growth factor-beta (TGF-beta) in fibroblasts. CCN proteins are characterized by a more than 10% cysteine content and an absolute conservation of the position of their 38 cysteine residues. Human CTGF is synthesized with a signal peptide sequence (a.a. 1-26), the removal of which yields the mature protein with an N-terminal IGF-binding protein (IGFBP) domain (a.a. 27-98), followed by a von Willebrand factor type C (VWC or VWFC) domain (a.a. 101-167), a cysteine-free hinge region (a.a. 168-197), thrombospondin type-1 (TSP-1) domain (a.a. 198-243), and a C-terminal cysteine-knot or C-terminal CYS-KNOT (CTCK) motif (a.a. 256-330). Due to the high number of disulfide bridges, most of the CTGF domains are resistant to proteolytic digestion, and most proteases primarily cleave only in the cysteine-free hinge region, producing almost exclusively intact N- and C-terminal half fragments. The N-terminal half of CTGF is shown to mediate differentiation and collagen synthesis in concert with IGF-2, while its C-terminal half mediates cell proliferation in concert with EGF.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified goat polyclonal antibody in buffer containing 0.3 M Glycine, 50 mM Tris, pH 8.0 without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-CTGF Antibody, N-Term Antibody is validated for use in Western Blotting, Neutralizing for the detection of CTGF.
      Key Applications
      • Western Blotting
      • Neutralizing
      Application NotesWestern Blotting Analysis: A representative lot detected the N-terminal (1-180), but not C-terminal (181-349), fragment derived from baculovirus expressed human CTGF by chymotrypsin cleavage. The N-terminal fragment appeared as a doublet due to differential glycosylation (Grotendorst, G.R., and Duncan, M.R. (2005). FASEB J. 19(7):729-738).
      Neutralizing Analysis: A representative lot inhibited CTGF-stimulated collagen synthesis, but not CTGF-stimulated DNA synthesis, in rat kidney (NRK) fibroblasts (Grotendorst, G.R., and Duncan, M.R. (2005). FASEB J. 19(7):729-738).
      Note: It is recommended that gel electrophoresis be performed under non-reducing condition to effectively separate CTGF N- and C-terminal fragments, as well as differentially glycosylated N-terminal fragments.
      Biological Information
      ImmunogenRecombinant full-length human CTGF.
      EpitopeN-terminal half.
      ConcentrationPlease refer to lot specific datasheet.
      HostGoat
      SpecificityThis polyclonal antibody was affinity-purified using the purified N-terminal fragment (1-180) from chymotrypsin-cleaved rhCTGF to eliminate immunoreactivity against the C-terminal half (181-349) of CTGF in the original goat antiserum raised against full-length rhCTGF (Grotendorst, G.R., and Duncan, M.R. (2005). FASEB J. 19(7):729-738).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CTGF
      • CCN2
      • HCS24
      • IGFBP8
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight35.92/38.09 kDa (isoform Long mature/pro-form), 18.95/16.35 kDa (isoform Long N-terminal fragment with/without a.a. 1-26 signal peptide), 33.04/35.21 kDa (isoform Short mature/pro-form) calculated. The N-terminal fragment (a.a. 1-180) generated by chymotrypsin cleavage of glycosylated CTGF can be resolved into a doublet under non-reducing condition with the upper band representing more glycosylated species.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting of chymotrypsin-cleaved CTGF fragments.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected 1 ng of the N-terminal (1-180), but not C-terminal (181-349), fragment derived from baculovirus expressed human CTGF by chymotrypsin cleavage.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABS1627 04055977352894

      Documentation

      Anti-CTGF Antibody, N-Term Antibody Certificates of Analysis

      TitleLot Number
      Anti-CTGF, N-Term - 3202214 3202214
      Anti-CTGF, N-Term - 3218761 3218761
      Anti-CTGF, N-Term - 3240480 3240480
      Anti-CTGF, N-Term - 3272234 3272234
      Anti-CTGF, N-Term - 3425375 3425375
      Anti-CTGF, N-Term - 4013440 4013440
      Anti-CTGF, N-Term -Q2690236 Q2690236
      Anti-CTGF, N-Term Polyclonal Antibody 3117819
      Anti-CTGF, N-Term Polyclonal Antibody 2907823
      Anti-CTGF, N-Term Polyclonal Antibody 3062434
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies