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MABF2296-25UG Anti-CX3CL1 Antibody, clone 16A10

Anti-CX3CL1, clone 16A10, Cat. No. MABF2296, is a mouse monoclonal antibody that detects CX3CL1 (Fractalkine) and is tested for use in Immunocytochemistry and Immunohistochemistry (Paraffin).

MABF2296-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2296-25UG
      DescriptionAnti-CX3CL1 Antibody, clone 16A10
      Alternate Names
      • Fractalkine
      • C-X3-C motif chemokine 1
      • CX3C membrane-anchored chemokine
      • Neurotactin
      • Small-inducible cytokine D1
      Background InformationFractalkine (UniProt: P78423; also known as C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, CXCL1) is encoded by the CXCL1 (also known as FKN, NTT, SCYD1, A-152E5.2) gene (Gene ID: 6376) in human. CX3CL1 is a monomeric, single-pass type I membrane protein that acts as a ligand for CX3CR1 and integrins. It is expressed in multiple tissues and the seminal plasma, endometrial fluid, and follicular fluid. Its highest levels have been reported in the brain and heart. It is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-341), a transmembrane domain (aa 342-362), and a cytoplasmic domain (aa 363-397). It can activate integrins in both a CX3CR1-dependent and independent manners. In the presence of CX3CR1, it activates integrins by binding to their classical ligand-binding site (site 1) and in the absence of CX3CR1, it binds to a second site (site 2) in integrins. Binding to site 2 enhances the binding of other integrin ligands to site 1. CX3CL1 can exist in bot soluble and membrane-bound form. The membrane-bound form is proteolytically cleaved by ADAM10 and ADAM17 to generate the soluble form. The soluble form is shown to be chemoattractive for T-cells and monocytes and the membrane-bound form promotes adhesion of those leukocytes to endothelial cells. The membrane-bound form can be induced on primary endothelial cells by inflammatory cytokines such as TNF- , interferon- (IFN- ), and interleukin-1. (Ref.: Jones, BA., et al, (2010). Mol Interv. 10(5); 263-270).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-CX3CL1, clone 16A10, Cat. No. MABF2296, is a mouse monoclonal antibody that detects CX3CL1 (Fractalkine) and is tested for use in Immunocytochemistry and Immunohistochemistry (Paraffin).
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected CX3CL1 in human brain tissue sections.

      Immunocytochemistry Analysis: 1:250 dilution from a representative lot detected CX3CL1 in U2OS cells.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenA recombinant fragment corresponding to 76 amino acids from the N-terminal, extracellular domain of human CX3CL1 (Fractalkine).
      EpitopeN-terminal, extracellular domain
      Clone16A10
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 16A10 is a mouse monoclonal antibody that detects human CX3CL1 (Fractalkine). It targets an epitope within 76 amino acids from the N-terminal, extracellular domain.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CXCL1
      • FKN
      • NTT
      • SCYD1
      • A-152E5.2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight42.20 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human testis tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected CX3CL1 in human testis tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABF2296-25UG 04061841990154