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MABF2107-25UG Anti-Chlamydial LPS Antibody, clone EVI-H1

Anti-Chlamydial LPS, clone EVI-H1, Cat. No. MABF2107, is a mouse monoclonal antibody that detects Chlamydial lipopolysaccharide (LPS) and has been tested for use in Electron Microscopy, Immunocytochemistry, Immunofluorescence, and Western Blotting.

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MABF2107-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2107-25UG
      DescriptionAnti-Chlamydial LPS Antibody, clone EVI-H1
      Alternate Names
      • Chlamydial lipopolysaccharide
      • Chlamydia trachomatis LPS
      Background InformationChlamydia trachomatis is a Gram-negative bacterium that is responsible to sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV). Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants. These are identified based on serological reactivity of the epitopes on their outer membrane. Intracellularly chlamydia replicates within a vacuole. Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles. This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell. Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate. Lipopolysaccharides (LPS) of Chlamydia consists of at least three antigen domains, two of which are shared by the LPS of certain other gram-negative organisms and one that is unique only to chlamydial LPS. It has been reported that the quantitative yields of chlamydial LPS can be achieved when elementary bodies (EBs) are first reduced and alkylated prior to extraction with hot phenol-water. (Ref.: Caldwell, HD., and Hitchcock, PJ. (1984). Infect. Immun. (44(2); 306-314; Scidmore, MA., et al. (1996). Infect. Immun. 64(12); 5366-5372; Turingan, RS et al. (2017). PLoS ONE 12(5): e0178653).
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Chlamydial LPS, clone EVI-H1, Cat. No. MABF2107, is a mouse monoclonal antibody that detects Chlamydial lipopolysaccharide (LPS) and has been tested for use in Electron Microscopy, Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Electron Microscopy
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).

      Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).

      Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).
      Biological Information
      ImmunogenFormalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.
      CloneEVI-H1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone EVH-H1 detects lipopolysaccharide from Chlamydia genus.
      IsotypeIgG2a
      Species Reactivity
      • Bacteria
      Species Reactivity NoteBacteria.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Molecular Weight~15 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cells infected with chlamydia trachomatis serovar L2 (LGV 434 L2).

      Western Blotting Analysis: 2 µg/mL of this antibody detected Chlamydial LPS in HeLa cells infected with chlamydia trachomatis serovar L2 (LGV 434 L2).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABF2107-25UG 04054839623530

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