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ABC481 Anti-Cytosolic 5'-nucleotidase II Antibody

Anti-Cytosolic 5'-nucleotidase II Antibody is an antibody against Cytosolic 5'-nucleotidase II for use in Western Blotting, Immunohistochemistry.

Anti-Cytosolic 5'-nucleotidase II Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: NT5C2, NT5B, NT5CP, PNT5 UniProt Number: P49902
ABC481
100 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC481
      DescriptionAnti-Cytosolic 5'-nucleotidase II Antibody
      Alternate Names
      • Cytosolic purine 5'-nucleotidase
      • Cytosolic 5'-nucleotidase II
      Background InformationCytosolic purine 5'-nucleotidase (EC 3.1.3.5; UniProt P49902; also known as 5'-nucleotidase (purine), cytosolic type B, Cytosolic 5'-nucleotidase II, IMP-specific 5'-NT, Purine 5' nucleotidase) is encoded by the NT5C2 (also known as cN-II, GMP, NT5B, NT5CP, PNT5, SPG45, SPG65) gene (Entrez Gene ID 22978) in human. NT5C2 protein is a cytosolic enzyme that catalyzes the hydrolysis of inosine 5'-monophosphate (IMP) and other purine nucleotides into the corresponding ribonucleoside and a phosphate. Purine nucleotides are neuroprotective and play a critical role in the ischemic and developing brain, alterations in purine nucleotides levels could sensitize neurons to stress and insult. Hereditary spastic paraplegias (HSPs) are neurodegenerative motor neuron diseases characterized by progressive age-dependent loss of corticospinal motor tract function. Mutations in NT5C2 and several other genes coding for purine nucleotide metabolizing enzymes are linked to autosomal recessive HSP (AR-HSP) in a study of 55 families displaying AR-HSP (PMID 24482476).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationAnti-Cytosolic 5'-nucleotidase II Antibody is an antibody against Cytosolic 5'-nucleotidase II for use in Western Blotting, Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Cytosolic 5'-nucleotidase II in human kidney tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Cytosolic 5'-nucleotidase II near the C-terminus.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Feline, Canine, Bovine, and Horse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NT5C2
      • NT5B
      • NT5CP
      • PNT5
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~65 kDa observed. This antibody is expected to detect both isoforms.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cytosolic 5'-nucleotidase II in 10 µg of A549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABC481 04055977168921