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MABE1011 Anti-DNA Ligase 3 Antibody, clone 7H6.1

This Anti-DNA Ligase 3 Antibody, clone 7H6.1 is validated for use in Western Blotting, Immunocytochemistry for the detection of DNA Ligase 3.

Anti-DNA Ligase 3 Antibody, clone 7H6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: LIG3 UniProt Number: P49916
MABE1011
100 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1011
      DescriptionAnti-DNA Ligase 3 Antibody, clone 7H6.1
      Alternate Names
      • DNA ligase 3
      • DNA Ligase 3
      • DNA ligase III
      • Polydeoxyribonucleotide synthase [ATP] 3
      Background InformationDNA ligase 3 (EC 6.5.1.1; UniProt P49916; also known as DNA ligase III, ATP-dependent polydeoxyribonucleotide synthase [ATP] 3) is encoded by the LIG3 gene (Gene ID 3980) in human. Three genes (LIG1, LIG3, and LIG4) in human genome encode ATP-dependent DNA ligases required for repairing DNA single- and double-strand breaks (SSB and DSB) and completing repair events initiated by the excision of mismatched and damaged bases. Among the human LIG genes, the LIG3 is unique in that it encodes multiple isforms with different N- and C-termini via alternative translation initiation and splicings. The "breast cancer susceptibility protein 1 C-terminal" (BRCT) domain present at the C-terminal end of the canonical isoform 1 (a.a. 933-1009) and isoform 3 is replaced by a short positively charged sequence that acts as a nuclear localization signal in spliced isoform 2 and isoform 4 (also known as the beta isoform). In addition, the N-terminal end of isoform 3 (also known as the alpha isoform) and isoform 4 do not contain the 87 a.a. mitochondrial targeting sequence present in isoform 1 and isoform 2. The LIG3 is the only gene encodes mitochondria DNA ligase (i.e. isoforms 1 & 2), inactivation of the LIG3 gene results in loss of mitochondrial function and cell death. Isoforms 1 and 3 interact with the scaffold protein XRCC1 via their BRCT domain, the XRCC1 in turn recruits a large number of proteins involved in base excision and single-strand break repair.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-DNA Ligase 3 Antibody, clone 7H6.1 is validated for use in Western Blotting, Immunocytochemistry for the detection of DNA Ligase 3.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected DNA Ligase 3 in HeLa, A431, HUVEC and NIH/3T3 cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the C-terminal of human DNA Ligase 3.
      EpitopeC-terminal
      Clone7H6.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with all four spliced isoforms.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LIG3
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~113/106 kDa observed. 112.9 kDa (isoform 1), 106.0 kDa (isoform 2), 102.7 kDa (isoform 3), 95.8 kDa (isoform 4) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected DNA Ligase 3 in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE1011 04055977297430