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GR15L Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

Overview

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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GR15L-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~170 kDa EGF receptor protein in A431 cells.
      Catalogue NumberGR15L
      Brand Family Calbiochem®
      SynonymsAnti-Epidermal Growth Factor Receptor
      Application Data
      Detection of human EGFR by immunocytochemistry. Sample: A431 cells fixed with methanol. Primary Antibody: Anti-Epidermal Growth Factor Receptor (Ab-5) Mouse mAb (Cat. No. GR15L) (1 µg/ml). Detection: fluorescence.
      References
      ReferencesHunter T., 1984. Nature 311, 414.
      Reynolds, F.H., Jr., et al. 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Negative controlHL-60 cells
      Positive controlA-431 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesNeutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511.
      Key Applications Flow Cytometry
      Immunofluorescence
      Immunoprecipitation
      Neutralization Studies
      Application NotesFlow Cytometry (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Neutralization Studies (1-2 µg/ml; see application references)
      Application CommentsStains A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. This antibody does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpartially purified, human EGFR from A431 cells
      ImmunogenHuman
      CloneEGFR.1
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
      Global Trade Item Number
      Catalog Number GTIN
      GR15L-100UG 04055977228250