05-104
Anti-EGFR Antibody, clone LA22
clone LA22, Upstate®, from mouse
Synonym(s):
Receptor tyrosine-protein kinase ErbB-1, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor, epidermal growth factor receptor (avian
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$444.00
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About This Item
$444.00
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
LA22, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... EGFR(1956)
1 of 4
This Item | 05-1047 | 09-310 | 06-847 |
|---|---|---|---|
| biological source mouse | biological source mouse | biological source rabbit | biological source rabbit |
| species reactivity human | species reactivity rat, mouse, human | species reactivity human | species reactivity rat, hamster, mouse, human |
| antibody form purified antibody | antibody form purified antibody | antibody form affinity isolated antibody | antibody form purified immunoglobulin |
| clone LA22, monoclonal | clone 8G6.2, monoclonal | clone polyclonal | clone polyclonal |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| technique(s) immunocytochemistry: suitable, western blot: suitable, immunoprecipitation (IP): suitable | technique(s) immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 1:2,000 using A431 cell lysate (used to detect EGFR), western blot: suitable | technique(s) ELISA: suitable, immunocytochemistry: suitable, western blot: suitable | technique(s) immunoprecipitation (IP): suitable, western blot: suitable |
General description
Specificity
Immunogen
Application
4 μg of a previous lot immunoprecipitated EGF receptor from 500 μg of A431 RIPA lysate.
Immunocytochemistry:
5 μg/mL of previous lots of the antibody showed positive immunostaining for EGF receptor in A431 cells fixed with 4% paraformaldehyde followed by 100% methanol.
Signaling
Growth Factors & Receptors
Quality
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected EGF receptor in RIPA lysates from human A431 cells under reducing conditions.
Target description
Linkage
Physical form
Storage and Stability
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Other Notes
Legal Information
Disclaimer
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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