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MABN2429-25UG Anti-GARP Antibody, clone 4B1

Anti-GARP, clone 4B1, Cat. No. MABN2429, is a highly specific mouse monoclonal antibody, that targets CNGB1 and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, Immunofluorescence and Western Blotting.

Research Category: Neuroscience Gene Symbol: CNGBI, CNCG2, CNCG3L, CNCG4, RCNC2 UniProt Number: Q14028
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MABN2429-25UG
25 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, BIH(P), IP, IF, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2429-25UG
      DescriptionAnti-GARP Antibody, clone 4B1
      Alternate Names
      • Cyclic nucleotide-gated cation channel beta-1
      • Cyclic nucleotide-gated cation channel 4
      • CNG channel 4
      • CNG-4
      • CNG4
      • Cyclic nucleotide-gated cation channel gamma
      • Cyclic nucleotide-gated cation channel modulatory subunit
      • Cyclic nucleotide-gated channel beta-1
      • CNG channel beta-1
      • Glutamic acid-rich protein
      • GARP
      Background InformationCyclic nucleotide-gated cation channel beta-1 (UniProt: Q14028; also known as Cyclic nucleotide-gated cation channel 4, CNG channel 4, CNG-4, CNG4, Cyclic nucleotide-gated cation channel gamma, Cyclic nucleotide-gated cation channel modulatory subunit, Cyclic nucleotide-gated channel beta-1, CNG channel beta-1, CNGB1, Glutamic acid-rich protein, GARP) is encoded by the CNGBI (also known as CNCG2, CNCG3L, CNCG4, RCNC2) gene in human. CNGB1 is a multi-pass membrane protein that is a subunit of cyclic nucleotide-gated (CNG) channels that are non-selective cation channels and play important roles in both visual and olfactory signal transduction. It forms a tetramer with three CNGA1 subunits. When associated with CNGA1, it is involved in the regulation of ion flow into the rod photoreceptor outer segment (ROS), in response to light-induced alteration of the levels of intracellular cGMP. CNGB1 contains one cyclic nucleotide-binding domain. Four isoforms of CNGB1 have been described that are generated by alternate splicing. Mutations in CNGB1 gene are associated with retinitis pigmentosa that is characterized by retinal pigment deposits that are visible on fundus examination and case loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-GARP, clone 4B1, Cat. No. MABN2429, is a highly specific mouse monoclonal antibody, that targets CNGB1 and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, Immunofluorescence and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected GARP in Western Blotting applications (Poetsch, A., et. al. (2001). J Biol Chem. 276(51):48009-16; Chakraborty, D., et. al. (2008). Biochemistry. 47(4):1144-56; Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).

      Immunofluorescence Analysis: A representative lot detected GARP in Immunofluorescence applications (Zhang, Y., et. al. (2009). J Cell Sci. 122(Pt 8):1192-200).

      Immunoprecipitation Analysis: A representative lot detected GARP in Immunoprecipitation applications (Conley, S.M., et. al. (2010). Adv Exp Med Biol. 664:63-70).
      Biological Information
      ImmunogenGST-tagged first 16 amino acids from the N-terminal region of human Cyclic nucleotide-gated cation channel beta-1.
      Clone4B1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4B1 detects Glutamic acid-rich protein (GARP) in mouse and bovine retina. It targets an epitope with in the first 16 amino acids from the N-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Bovine
      Species Reactivity NoteMouse, Bovine. Predicted to react with Human and Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • CNGBI
      • CNCG2
      • CNCG3L
      • CNCG4
      • RCNC2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight139.68 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in mouse retina tissue.

      Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected GARP in mouse retinal tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABN2429-25UG 04054839512490

      Documentation