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ABC945 Anti-GMPR Antibody

This Anti-GMPR Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of GMPR.

Anti-GMPR Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Cytoskeletal Signaling Gene Symbol: GMPR, GMPR1 UniProt Number: P36959
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ABC945
100 μg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC945
      DescriptionAnti-GMPR Antibody
      Alternate Names
      • GMP reductase 1
      • Guanosine 5'-monophosphate oxidoreductase 1
      • Guanosine monophosphate reductase 1
      Background InformationGMP reductase 1 (EC 1.7.1.7; UniProt P36959; also known as Guanosine 5'-monophosphate oxidoreductase 1, Guanosine monophosphate reductase 1) is encoded by the GMPR (also known as GMPR1) gene (Gene ID 2766) in human. De novo biosynthesis of Guanosine 5'-monophosphate (GMP) is accomplished by reactions catalyzed by several enzymes. Inositol monophosphate (IMP) dehydrogenase 1 and 2 (IMPDH1 and IMPDH2) convert IMP into xanthosine monophosphate (XMP), GMP synthetase (GMPS) then converts XMP into GMP, while guanosine monophosphate reductase (GMPR) catalyzes the reversible conversion of GMP to IMP to fuel back into both the AMP and GMP synthesis pathways. GMPR is shown to reduce intracellular GTP pools, thereby downregulating the level of several GTP-bound (active) RHO-GTPases that are essential for invadopodia formation, extracellular matrix degradation and invasion of melanoma cells both in cultures in vitro and in tumor xenografts in vivo. Consistent with its role as a negative regulator of the invasive melanoma phenotype, GMPR expression is reported to be either undetectable or drastically reduced in human melanoma cell lines when compared to normal melanocytes.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-GMPR Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of GMPR.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected GMPR in human prostate cancer, as well as in normal testis and skeletal muscle tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding a sequence between the second and third GMP-binding region of human GMPR.
      EpitopeBetween the second and third GMP-binding region.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects human GMP reductase 1 (GMPR) and stained predominantly the cytoplasm of cells in human tissue sections by immunohistochemistry.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Primate, Bovine, Mouse, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GMPR
      • GMPR1
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight ~35 kDa observed. 37.42 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human skeletal muscle tissue lysate.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected GMPR in 10 µg of human skeletal muscle tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABC945 04055977352825