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MAB10209-I-25UL Anti-HSV-2 Nucleocapsid p40 Antibody, clone 3E1

Anti-HSV-2 Nucleocapsid p40, clone 3E1, Cat. No. MAB10209-I, is a mouse monoclonal antibody that detects HSV-2 Nucleocapsid p40 and is tested for use in Immunohistochemistry (Paraffin).

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MAB10209-I-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB10209-I-25UL
      DescriptionAnti-HSV-2 Nucleocapsid p40 Antibody, clone 3E1
      Alternate Names
      • Capsid scaffolding protein
      • Capsid protein P40
      • Protease precursor
      • pPR
      • Virion structural protein UL26
      Background InformationCapsid scaffolding protein (UniProt: P89449; also known as Capsid protein P40, Nucleocapsid protein P40, Protease precursor, pPR, Virion structural protein UL26) is encoded by the UL26 gene (Gene ID: 1487310) in Herpes Simplex virus 2 (HSV-2). HSV-2 is a large complex DNA-containing virus that is shown to induce the synthesis of at least 50 new polypeptides in infected cells. It replicates in the nuclei of mammalian cells. Its nuclear localization signal is localized in amino acids 428-431. Following its synthesis, Capsid protein 40 is processed into Assemblin (aa 1-237), and Assembly protein (aa 248-637) by proteolytic cleavage. Assemblin is a protease that plays an essential role in virion assembly within the nucleus. It exists in a monomer-dimer equilibrium with the dimer being the active species. It catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The assembly protein is involved in capsid assembly. It acts as a scaffold protein by binding major capsid protein and multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. It is cleaved by Assemblin after capsid completion and the cleavages products are evicted from the capsid before or during DNA packaging. HSV is reported to evade the immune system through interference with MHC class I antigen presentation on the cell surface, by blocking the transporter associated with antigen processing (TAP) induced by the secretion of infected cell protein 47 (ICP-47) by HSV. ICP-47 prevents initiation of a CTL-response against HSV, allowing the virus to survive for a protracted period in the host. (Ref.: Goldsmith, K., et a. (1998). J. Exp. Med. 187(3); 341-348; Heilman, CJ., et al. (1981). J. Virol. 40(2); 508-515).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-HSV-2 Nucleocapsid p40, clone 3E1, Cat. No. MAB10209-I, is a mouse monoclonal antibody that detects HSV-2 Nucleocapsid p40 and is tested for use in Immunohistochemistry (Paraffin).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Biological Information
      ImmunogenPurified HSV-2 nucleocapsids.
      EpitopeUnknown
      Clone3E1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3E1 is a mouse monoclonal antibody that detects nucleocapsid protein p40 in Herpes Simplex virus 2
      IsotypeIgG1
      Species Reactivity
      • Herpes Simplex Virus
      Species Reactivity NoteHerpes simplex virus.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • UL26
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight66.94 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in HSV-2-positive tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected HSV-2 Nucleocapsid p40 in HSV-2-positive tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB10209-I-25UL 04065267027777