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MABS1907-100UG Anti-Ketohexokinase Isoform C Antibody, clone 5F1-3F5

Anti-Ketohexokinase Isoform C, clone 5F1-3F5, Cat. No. MABS1907, is a mouse monoclonal antibody that detectsKetohexokinase and has been tested for use in Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: KHK UniProt Number: P50053-1
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MABS1907-100UG
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1907-100UG
      DescriptionAnti-Ketohexokinase Isoform C Antibody, clone 5F1-3F5
      Alternate Names
      • EC: 2.7.1.3
      • Hepatic fructokinase
      • Fructokinase
      Background InformationKetohexokinase (UniProt: P50053; also known as EC: 2.7.1.3, Hepatic fructokinase) is encoded by the KHK gene (Gene ID: 3795) in human. Ketohexokinase is a homodimeric cytosolic enzyme in that catalyzes the conversion of fructose to fructose 1 phosphate. It is most abundant in the liver, where it may constitute up to 0.6% of the total liver protein. It has also been reported in kidney, spleen, gut, and pancreas. Very low levels are also found in adrenal, muscle, brain, and eyes. It has multiple fructose binding sites. There are two major isoforms of this enzyme ketofructokinase C and ketofructokinase A. Due to its high Km (~ 28 mM), fructose is preferentially metabolized by the C isoform. Ketohexokinase -C is unique among sugar kinases in that its metabolism of fructose is associated with a rapid depletion of intracellular ATP. Unlike glucokinase, in which excessive phosphorylation of glucose is prevented by a negative feedback system, the metabolism of fructose by ketohexokinase-C results in rapid phosphorylation with a fall in intracellular phosphate and ATP. If the ATP levels fall significantly then the protein synthesis is transiently stopped and cells may develop features that are consistent with ischemia. Mutations in KHK gene has been linked to fructosuria, a clinically benign condition that is characterized nu the incomplete metabolism of fructose in the liver, leading to its urinary excretion. (Ref.: Diggle, CP et al., (2009). J. Histochem. Cytochem. 57(8): 763-774; Hayward, BE., and Bonthron, DT (1998). Eur. J. Biochem. 257(1); 85-91).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Ketohexokinase Isoform C, clone 5F1-3F5, Cat. No. MABS1907, is a mouse monoclonal antibody that detectsKetohexokinase and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 2 µg/mL from a represenative lot detected Ketohexokinase Isoform C in HEK293 cells expressing HA-tagged KHK-C.

      Western Blotting Analysis: 1 µg/mL from a represenative lot detected Ketohexokinase Isoform C in human liver tissue lysate.
      Biological Information
      ImmunogenA full-length human recombinant Ketohexokinase, isoform C.
      Clone5F1-3F5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5F1-3F5 specifically detects isoform C of human ketohexokinase.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KHK
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~33 kDa observed; 32.52 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human kidney tissue lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Ketohexokinase Isoform C in human kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS1907-100UG 04054839563287