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MABC1108-25UG Anti-LAMP-1 Antibody, clone H4A3

Anti-LAMP-1, clone H4A3, Cat. No. MABC1108, is a mouse monoclonal antibody that detects Lysosome-associated membrane glycoprotein 1 (LAMP-1) and has been tested for use in Dot Blot, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-LAMP-1 Antibody, clone H4A3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: LAMP1 UniProt Number: P11279
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MABC1108-25UG
25 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HDB, IF, IH(P), IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1108-25UG
      DescriptionAnti-LAMP-1 Antibody, clone H4A3
      Alternate Names
      • Lysosome-associated membrane glycoprotein 1
      • Lysosome-associated membrane protein 1
      • CD107 antigen-like family member A
      • CD107a
      Background InformationLysosome-associated membrane glycoprotein 1 (UniProt: P11279; also known as LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a) is encoded by the LAMP1 gene (Gene ID: 3916) in human. LAMP-1 and LAMP-2 are two highly glycosylated proteins found in the lysosomal membrane. They may contribute up to 50% of all proteins present in the lysosomal membranes. They are known to maintain the lysosomal acidification and lysosomal membrane integrity. Extensive N-glycosylation on LAMP-1 and 2 to protects them from the lysosomal proteolysis. However, higher levels of poly-N-acetyllactosamines on LAMP-1 and 2 have been correlated with decreased cellular differentiation and enhanced metastatic potential. LAMP-1 is a single-pass type I transmembrane protein that contains a large lumenal domain (aa 29-382), a transmembrane domain (aa 383-405), and a short C-terminal cytoplasmic tail (aa 406-417). LAMP-1 is synthesized with a signal peptide (aa 1-28) that is subsequently cleaved in the mature form. LAMP-1 presents carbohydrate ligands to selectins and is also reported to act as a receptor for Lassa virus protein. Two isoforms of LAMP-1 have been described that are produced by alternative splicing. (Ref.: Tan, K-P., et al. (2016). Cell Death Dis. 7, e2347).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-LAMP-1, clone H4A3, Cat. No. MABC1108, is a mouse monoclonal antibody that detects Lysosome-associated membrane glycoprotein 1 (LAMP-1) and has been tested for use in Dot Blot, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Dot Blot
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected LAMP-1 in HeLa and A431 cells.

      Immunohistochemistry (Paraffin) Analysis: A representative lot detected LAMP-1 in Immunohistochemistry applications (Furuta, K., et. al. (2001). Am J Pathol. 159(2):449-55).

      Western Blotting Analysis: A representative lot detected LAMP-1 in Western Blotting applications (Mane, S.M., et. al. (1989). Arch Biochem Biophys. 268(1):360-78).

      Immunofluorescence Analysis: A representative lot detected LAMP-1 in Immunofluorescence applications (Starr, T., et. al. (2011). Cell Host Microbe. 11(1):33-45).

      Dot Blot Analysis: A representative lot detected LAMP-1 in Dot Blot applications (Arruda, L.B., et. al. (2006). J Immunol. 177(4):2265-75).

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected LAMP-1 in human kidney and human pancreas tissue sections.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated LAMP-1 in Immunoprecipitation applications (Mane, S.M., et. al. (1989). Arch Biochem Biophys. 268(1):360-78).
      Biological Information
      ImmunogenHuman adherent peripheral blood cells.
      CloneH4A3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone H4A3 detects Lysosome-associated membrane glycoprotein 1 (LAMP-1) in human cells.
      IsotypeIgGκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LAMP1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~110 kDa observed; 44.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected LAMP-1 in HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABC1108-25UG 04054839516832