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04-1556 Anti-MDM4 Antibody, clone 7A8

Use Anti-MDM4 Antibody, clone 7A8 (mouse monoclonal antibody) validated in WB to detect MDM4 also known as Double minute 4 protein, MDM4-related protein 1, Mdm2-like p53-binding protein, Mdm4 p53 binding protein homolog (mouse).

Anti-MDM4 Antibody, clone 7A8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Transcription Factors, Apoptosis - Additional Gene Symbol: MDM4, HDMX, MDMX, MRP1 UniProt Number: O35618
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04-1556
100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1556
      DescriptionAnti-MDM4 Antibody, clone 7A8
      Alternate Names
      • Double minute 4 protein
      • MDM4-related protein 1
      • Mdm2-like p53-binding protein
      • Mdm4 p53 binding protein homolog (mouse)
      • Mdm4, transformed 3T3 cell double minute 4, p53 binding
        protein
      • Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)
      • Protein Mdmx
      • double minute 4, human homolog of
      • p53-binding protein
      • mouse double minute 4 homolog
      • mouse double minute 4, human homolog of
      • p53-binding protein
      • p53-binding protein
      • p53-binding protein Mdm4
      Background InformationMDM4 (sometimes referred to as MDMX) is a member of the MDM family proteins. It is known to inhibit p53 and p73 mediated cell cycle arrest and apoptosis. MDM4 is amplified or overexpressed in 10-20% of tumors and 65% of retinoblastomas. The full-length protein has significant homology to MDM2 and can also bind to and inhibit p53. In both MDM2 and MDM4, this interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Other similar domains include the C-terminal RING finger motif. Although these proteins share similar domains, MDM2 and MDM4 are non-redundant p53 inhibitors, since normal levels of either protein does not compensate for the loss of the other. Recent evidence suggests complementary activities for these proteins with MDM4 regulating p53 activity, and MDM2 playing a key role in p53 stability.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-MDM4 Antibody, clone 7A8 (mouse monoclonal antibody) validated in WB to detect MDM4 also known as Double minute 4 protein, MDM4-related protein 1, Mdm2-like p53-binding protein, Mdm4 p53 binding protein homolog (mouse).
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to mouse MDM4.
      EpitopeUnknown
      Clone7A8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes MDM4.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MDM4
      • HDMX
      • MDMX
      • MRP1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions. The short isoform is a more potent inhibitor of p53 activity than the long isoform.

      SUBUNIT STRUCTURE: Binds to p53, p73 and MDM2.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: In all tissues tested. The short isoform is expressed in a variety of transformed cell lines.

      DOMAIN: Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.

      SEQUENCE SIMILARITIES: Belongs to the MDM2/MDM4 family.

      Contains 1 RanBP2-type zinc finger.

      Contains 1 RING-type zinc finger.

      Contains 1 SWIB domain.
      Molecular Weight~ 80/27 kDa Observed. There are multiple forms for MDM4. Up to 7 different variants have been reported. Full legth of MDM4 is ~80kDa. The short form of MDM4 (MDM4-S) migrates at approximately 17 and 27kDa. (R. Rallapalli, G. Strachan, B. Cho, W. Mercer‡, and DJ. Hall, 1999, A Novel MDMX Transcript, THE JOURNAL OF BIOLOGICAL CHEMISTRY, 274(12), 8299–8308)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM4 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      04-1556 04053252741784

      Documentation

      Anti-MDM4 Antibody, clone 7A8 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MDM4 Antibody, clone 7A8 Certificates of Analysis

      TitleLot Number
      Anti-MDM4, clone 7A8 3073298
      Anti-MDM4, clone 7A8 - 2373429 2373429
      Anti-MDM4, clone 7A8 - 2427493 2427493
      Anti-MDM4, clone 7A8 - 2443057 2443057
      Anti-MDM4, clone 7A8 - 1981630 1981630
      Anti-MDM4, clone 7A8 - 2168278 2168278
      Anti-MDM4, clone 7A8 - 2343597 2343597
      Anti-MDM4, clone 7A8 - 3209742 3209742
      Anti-MDM4, clone 7A8 - 3698146 3698146
      Anti-MDM4, clone 7A8 - 3928112 3928112
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