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ABF1059 Anti-MxB Antibody

This Anti-MxB Antibody is validated for use in Western Blotting for the detection of MxB.

Anti-MxB Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: MX2 UniProt Number: P20592
ABF1059
100 µL  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABF1059
      DescriptionAnti-MxB Antibody
      Alternate Names
      • Interferon-induced GTP-binding protein Mx2
      • Interferon-regulated resistance GTP-binding protein MxB
      • Myxovirus resistance protein 2
      • p78-related protein
      • MxB
      Background InformationInterferon-induced GTP-binding protein Mx2 (UniProt P20592; also known as Interferon-regulated resistance GTP-binding protein MxB, Myxovirus resistance protein 2, p78-related protein, Second interferon-induced protein p78) is encoded by the MX2 (also known as MXB) gene (Gene ID 4600) in human. The interferon-inducible myxovirus resistance (Mx) proteins belong to the family of large GTPases and are highly homologous with dynamins within their GTP-binding domain. MxA/Mx1 is cytosolic, while two MxB/Mx forms exist, a 78 kDa nuclear form and a 76 kDa cytosolic form lacking the N-terminal nuclear localization signal (NLS). Human MxA protein mediates resistance to influenza and several other viruses, while human MxB is also reported to inhibit HIV-1 infection by reducing the level of integrated viral DNA. In addition, HIV-1 with Ala88 mutation in the capsid protein (CA) is found to escape MxB restriction, as a result of impaired CA interaction with the host peptidylprolyl isomerase cyclophilin A (CypA). Likewise, CypA deletion results in the loss of MxB inhibition of HIV-1.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-MxB Antibody is validated for use in Western Blotting for the detection of MxB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected IFNalpha-2b-induced MxB/Mx2 expression in U87-MG human glioblastoma cells and SupT1 human T lymphoblast cells (Liu, Z., et al. (2013). Cell Host Microbe. 14(4):398-410).
      Western Blotting Analysis: A representative lot detected doxycycline-induced MxB/Mx2 expression using virally transformed human T lymphoblast SupT1 cells that stably expresses the reverse tetracycline-controlled transactivator (rtTA) protein and carries the MxB gene under the tetracycline-responsive promoter element/TRE (Liu, Z., et al. (2013). Cell Host Microbe. 14(4):398-410).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the Dynamin-type G domain of human MxB.
      EpitopeDynamin-type G domain
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityExpected to react with both spliced isoforms.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Primate based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MX2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~78 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in IFN alpha 2b treated SupT1 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antiserum detected IFNalpha-2b-induced MxB/Mx2 expression in human T lymphoblast SupT1 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABF1059 04055977288001

      Documentation

      Anti-MxB Antibody Certificates of Analysis

      TitleLot Number
      Anti-MxB - 3683786 3683786
      Anti-MxB -Q2607332 Q2607332

      References

      Reference overviewPub Med ID
      The interferon-inducible MxB protein inhibits HIV-1 infection.
      Liu, Z; Pan, Q; Ding, S; Qian, J; Xu, F; Zhou, J; Cen, S; Guo, F; Liang, C
      Cell host & microbe  14  398-410  2013

      Show Abstract       		24055605 24055605