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MABF2755-25UL Anti-Myeloid lineage Antibody, clone OX-82

Anti-Myeloid lineage, clone OX-82, Cat. No. MABF2755, is a mouse monoclonal antibody that detests cells of the Myeloid lineage and is tested for use in Flow Cytometry and Fluorescence Activated Cell Sorting (FACS),

MABF2755-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2755-25UL
      DescriptionAnti-Myeloid lineage Antibody, clone OX-82
      Background InformationHematopoietic stem cells (HSC) are known to differentiate either into lymphoid cells or and myeloid cells that include granulocytes, monocytes, megakaryocytes, and dendritic cells. During hematopoiesis, lymphoid lineage cells originate from the lymph tissue and myeloid lineage cells originate from the bone marrow. Hematopoiesis is shown to change over time, with a bias of the dominating HSC towards myeloid development with advancing age. Defects in myelopoiesis is reported to result in several hematologic disorders. Clone OX-82 identifies myeloid cells and stromal elements in a variety of tissues in the adult. It reacts with a 35 kDa molecule expressed on myeloid cells and is useful in isolating pluripotent stem cells from rat bone marrow. (Ref.: Weiskopf, K., et al. (2016). Microbiol Spectr. 4(5); doi:10.1128; Crook, K., and Hunt, SV. (1996). Dev. Immunol. 4(4); 235-246).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Myeloid lineage, clone OX-82, Cat. No. MABF2755, is a mouse monoclonal antibody that detests cells of the Myeloid lineage and is tested for use in Flow Cytometry and Fluorescence Activated Cell Sorting (FACS),
      Key Applications
      • Flow Cytometry
      • Fluorescence Activated Cell Sorting (FACS)
      Application NotesTested applications

      Fluorescence Activated Cell Sorting (FACS): A representative lot detected Myeloid lineage in Fluorescence Activated Cell Sorting applications (Crook, K., et al. (1996). Dev Immunol. 4(4):235-46).

      Flow Cytometry Analysis: A representative lot detected Myeloid lineage in Flow Cytometry applications (Crook, K., et al. (1996). Dev Immunol. 4(4):235-46; Shaaban, S., et al. (2016). Int J Cancer Res. 12(2):69-81).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenAnemic rat bone marrow cells.
      EpitopeExtracellular domain
      CloneOX-82
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone OX-82 is a mouse monoclonal antibody that detects Myeloid lineage cells.
      IsotypeIgG1
      Species Reactivity
      • Rat
      Species Reactivity NoteRat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in Rat neutrophils.

      Flow Cytometry Analysis: 1.0 µg of this antibody detected Myeloid lineage in one million Rat neutrophils.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABF2755-25UL 04065270172495