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MABC182 Anti-NRF-1 Antibody, clone 10B11.1

This Anti-NRF-1 Antibody, clone 10B11.1 is validated for use in Western Blotting for the detection of NRF-1.

Anti-NRF-1 Antibody, clone 10B11.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Transcription Factors Gene Symbol: NRF1, ALPHA-PAL UniProt Number: Q16656
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MABC182
100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC182
      DescriptionAnti-NRF-1 Antibody, clone 10B11.1
      Alternate Names
      • Nuclear respiratory factor 1
      • Alpha-pal
      • Alpha palindromic-binding protein
      • NRF-1
      Background InformationNuclear respiratory factor 1 (UniProt Q16656; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the NRF1 (Also known as ALPHA-PAL) gene (Gene ID 4899) in human. NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS), resulting in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-NRF-1 Antibody, clone 10B11.1 is validated for use in Western Blotting for the detection of NRF-1.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant human NRF-1 internal fragment.
      EpitopeInternal (N-terminal half).
      Clone10B11.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityImmunogen construct region is present in human NRF-1 spliced isoform Long, isfoform Short, and isfoform 4, but is more than 40% absent from spliced isoform 4 reported by UniProt (Q16656).
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NRF1
      • ALPHA-PAL
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~58 kDa observed. 53.54/53.57 kDa (human/mouse isoform Long), 46.16 kDa (human/mouse isoform Short), 36.05 kDa (human isoform 3), 55.67 kDa (human isoform 4) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

      Western Blotting Analysis: 0.25 µg/mL of this antibody detected NRF-1 in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABC182 04055977355338

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies