This product is a polyclonal antibody that was made against biochemically isolated NF-H purified from bovine spinal cord. This preparation is dominated by axonal forms of NF-H which are heavily phosphorylated on the multiply repeated NF-H KSP type sequences, and this antibody reacts very strongly with these phosphorylated repeats. Reactivity with non-phosphorylated KSP sequences is orders of magnitude weaker. In most species there is some cross-reactivity with the phosphorylated KSP sequences found in the related neurofilament subunit NF-M which are similar but not identical to those of NF-H. The antibody recognizes phosphorylated NF-H strongly in all mammals tested to date.
AB5539
Anti-Neurofilament H (200 kDa) Antibody
CHEMICON®, chicken polyclonal
Synonym(s):
NF-H
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50 μL
$577.00
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
$577.00
Check Cart for Availability
A recombinant, preservative-free antibody is available for your target. Try ZRB1347
Request a Bulk Order
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Product Name
Anti-Neurofilament H Antibody, Chemicon®, from chicken
biological source
chicken
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, pig, bovine, feline, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
bovine ... Nefh(528842)
cat ... Nefh(101087272)
human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)
Specificity
Recognizes Neurofilament-Heavy (NF-H). The antibody stains sharply defined cytoplasmic filaments in neuronal cell bodies and especially axons.
Immunogen
Purified bovine NF-H.
Application
Anti-Neurofilament H Antibody is an antibody against Neurofilament for use in IC, IH, IH(P) & WB.
Western blot: 1:1,000-1:10,000 using ECL. Recognizes a band of 200-220 kDa.
Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.
Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.
Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Optimal working dilutions must be determined by the end user.
Target description
200 kDa
Physical form
Format: Purified
Purified chicken IgY in PBS containing 5mM sodium azide.
Analysis Note
Control
Positive control: brain.
Negative control: kidney, liver.
Positive control: brain.
Negative control: kidney, liver.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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