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MABS2299-25UG Anti-Neuropilin-1 Antibody, ECD b1b2 Antibody, clone mAb3

Anti-Neuropilin-1, ECD b1b2, clone mAb3, Cat. No. MABS2299, is a mouse monoclonal antibody that detects Neuropilin-1 (NRP1) and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Inhibition Assay.

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MABS2299-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2299-25UG
      DescriptionAnti-Neuropilin-1 Antibody, ECD b1b2 Antibody, clone mAb3
      Alternate Names
      • Vascular endothelial cell growth factor 165 receptor
      • CD304
      Background InformationNeuropilin-1 (UniProt: O14786; also known as Vascular endothelial cell growth factor 165 receptor, CD304) is encoded by the NRP1 (also known as NRP, VEGF165R) gene (Gene ID: 8829) in human. Neuropilins are highly conserved, non-tyrosine kinase, single-transmembrane glycoproteins that serve as co-receptors for various molecules, including semaphorins and vascular endothelial growth factors (VEGF). Neuropilin-1 is a single-pass type I, homodimeric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 22-856), a transmembrane domain (aa 857-879), and a cytoplasmic domain (aa 880-923). It acts as a receptor involved in the development of the cardiovascular system, angiogenesis, and formation of certain neuronal circuits. It is also reported to mediate the chemorepulsant activity of semaphorins. Neuropilin-1 has four well conserved domains: CUB (a1/a2; aa 27-141 and 147-265) domain, F5/8 (b1/b2; aa 275-424 and 431-583) domain, MAM domain (aa 645-811), and another domain that contains a transmembrane and a short cytoplasmic region. CUB domains mediate binding to semaphorins, while the tandem F5/8 domains are responsible for VEGF and heparin binding and recognizes a C-end role (CendR) motif R/KXXR/K on its ligands. The presence of the CUB (a1a2) domain is reported to enhance VEGF165 binding to F5/8 (b1b2) domain. The MAM domain is reported to mediate semaphorin signaling to Plexin-A. Neuropilin-1 has been recognized as an additional cellular mediator that facilitates the entry of SARS-CoV-2 into host cells. It binds to furin-cleaved spike protein and significantly potentiates SARS-CoV-2 infectivity. This effect can be blocked by monoclonal antibodies against the extracellular F5/8 (b1/b2) domain of Neuropilin-1 or with selective Neuropilin antagonists that bind to CendR binding b1 pocket. (Ref.: Kyrou, I., et al. (2021). Signal Trans. Targeted Ther. 6; Article number: 21; Daly, JL., et al. (2020). Science. 370(6518); 861-865; Cantuti-Castelvetri, L., et al. (2020). Science. 370(6518); 856-860; Mamluk, R., et al. (2002). J. Biol. Chem. 277(27); 24818-24825).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Neuropilin-1, ECD b1b2, clone mAb3, Cat. No. MABS2299, is a mouse monoclonal antibody that detects Neuropilin-1 (NRP1) and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Inhibition Assay.
      Key Applications
      • Immunocytochemistry
      • ELISA
      • Immunofluorescence
      • Inhibition
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected Neuropilin-1 in Immunofluorescence applications (Cantuti-Castelvetri, L., et al. (2020). Science. 370(6518):856-860; Daly, J.L., et al. (2020). Science. 370(6518):861-865).

      Immunocytochemistry Analysis: A representative lot detected Neuropilin-1 in Immunocytochemistry applications (Daly, J.L., et al. (2020). Science. 370(6518):861-865).

      Inhibition: A representative lot detected Neuropilin-1 in Inhibition applications (Cantuti-Castelvetri, L., et al. (2020). Science. 370(6518):856-860; Daly, J.L., et al. (2020). Science. 370(6518):861-865).

      ELISA Analysis: A representative lot detected Neuropilin-1 in ELISA applications (Daly, J.L., et al. (2020). Science. 370(6518):861-865).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 311 amino acids from the b1b2 region within the extracellular domain of human Neuropilin-1.
      EpitopeExtracellular domain
      ClonemAb3 (alternatively 7E8)
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone mAb3 is a mouse monoclonal antibody that detects Neeuropilin-1. It targets an epitope within the b1b2 domain in the extracellular region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NRP1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight103.13 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in PC-3 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Neuropilin-1 in PC-3 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS2299-25UG 04065267613956

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