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MABS818 Anti-PRKAA1, clone 2B7 Antibody

Detect PRKAA1 using this Anti-PRKAA1, clone 2B7 Antibody validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Flow Cytometry and ELISA.

Anti-PRKAA1, clone 2B7 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: PRKAA1, AMPK1 UniProt Number: Q13131
MABS818
100 µL  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IHC, ICC, FC, ELISAMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABS818
      Replaces04-323
      DescriptionAnti-PRKAA1, clone 2B7 Antibody
      Alternate Names
      • 5'-AMP-activated protein kinase catalytic subunit alpha-1
      • AMPK subunit alpha-1
      • PRKAA1
      Background Information5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1) belongs to the ser/thr protein kinase family. PRKAA1 is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
      References
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG1ascitic fluid containing 0.03% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationDetect PRKAA1 using this Anti-PRKAA1, clone 2B7 Antibody validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Flow Cytometry and ELISA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      • Flow Cytometry
      • ELISA
      Application NotesImmunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected PRKAA1 in ovarian cancer and brain tissues.

      Immunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected PRKAA1 in Ntera-2 cells.

      Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected PRKAA1 in PC-2 cells.

      ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
      Biological Information
      ImmunogenPurified recombinant fragment of human PRKAA1 expressed in E. Coli.
      Clone2B7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • PRKAA1
      • AMPK1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~65 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH/3T3, K562, HEK293, and PC-12 cell lysates.

      Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected PRKAA1 in Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH/3T3, K562, HEK293, and PC-12 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS818 04055977172362

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies