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MABE629 Anti-PRKDC, clone 3H6 Antibody

Anti-PRKDC, clone 3H6 Antibody is an antibody against PRKDC for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, ELISA.

Anti-PRKDC, clone 3H6 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: PRKDC, HYRC, HYRC1 UniProt Number: P78527
MABE629
100 µL  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, ICC, ELISAMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABE629
      Replaces04-1024
      DescriptionAnti-PRKDC, clone 3H6 Antibody
      Alternate Names
      • DNA-dependent protein kinase catalytic subunit
      • DNA-PK catalytic subunit
      • DNA-PKcs
      • PRKDC
      Background InformationDNA-dependent protein kinase catalytic subunit (DNA-PK catalytic subunit or DNA-PKcs), also known as DNPK1 or p460, and encoded by the gene name PRKDC or HYRC, is a nuclear protein kinase that is involved in DNA nonhomologous end joining (NHEJ) required to protect and align broken ends of DNA and V(D)J recombination. DNA-PK/PRKDC must be bound to DNA to express its catalytic properties. Autophosphorylation of DNA-PK/PRKDC correlates with loss of protein kinase activity and dissociation of the DNA-PKcs-Ku complex. DNA-PK/PRKDC phosphorylates Hsp90, p53 and c-Jun.
      References
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationAnti-PRKDC, clone 3H6 Antibody is an antibody against PRKDC for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, ELISA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      • ELISA
      Application NotesImmunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected PRKDC in breast cancer, colon cancer, lung cancer, and brain tissues.

      Immunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected PRKDC in HeLa cells.

      ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
      Biological Information
      ImmunogenPurified recombinant fragment of human PRKDC expressed in E. Coli.
      Clone3H6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • PRKDC
      • HYRC
      • HYRC1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~47 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 and PRKDC -- hIgGFc transfected HEK293 cell lysates.

      Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected PRKDC in HEK293 and hIgGFc transfected HEK293 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE629 04055977171730

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies