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MABS480 Anti-PTPN22 Antibody, clone 3B3.1

This Anti-PTPN22 Antibody, clone 3B3.1 is validated for use in Western Blotting for the detection of PTPN22.

Anti-PTPN22 Antibody, clone 3B3.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: PTPN22, PTPN8 UniProt Number: Q9Y2R2
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MABS480
100 µL  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMABS480
      DescriptionAnti-PTPN22 Antibody, clone 3B3.1
      Alternate Names
      • Tyrosine-protein phosphatase non-receptor type 22
      • Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP
      • Lymphoid phosphatase
      • LyP
      • PEST-domain phosphatase
      • PEP
      Background InformationTyrosine-protein phosphatase non-receptor type 22 (EC:3.1.3.48; UniProt Q9Y2R2; also known as Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, Lymphoid phosphatase, Lymphoid-specific protein tyrosine phosphatase, Lyp, PEP, PEST-domain phosphatase, Protein tyrosine phosphatase non-receptor type 8) is encoded by the PTPN22 (also known as LYP, LYP1, LYP2, PTPN8) gene (Gene ID 26191) in human. PTPN22/Lyp is expressed in all hematopoietic cells and belongs to the PEST group of nonreceptor classical class I protein tyrosine phosphatases (PTPs). Lyp is a negative regulator of TCR signal transduction by targeting phosphorylated Lck (Y394), Fyn (Y427), and Zap-70, as well as phospho-sites on TCRζ, CD3ε, Vav, and Vcp. Lyp utilizes its proline-rich region 1 to interact with the SH3 domain of C-terminal Src kinase (Csk), resulting in enhanced inhibitory tuning of Src family kinases and TCR signal transduction. TRAF3 is also shown to bind the proline-rich region 1 of Lyp in myeloid cells and impact its turnover. Additional binding partners of Lyp include the adaptor molecule Grb2, the E3 ligase c-Cbl.
      References
      Product Information
      FormatCulture Supernatant
      PresentationMouse monoclonal IgG1κ in supernatant without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-PTPN22 Antibody, clone 3B3.1 is validated for use in Western Blotting for the detection of PTPN22.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the C-terminal of human PTPN22.
      EpitopeC-terminal
      Clone3B3.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3B3.1 is expected to react with spliced isoform 1 (LyP1), isoform 3, and isoform 4 (LyP3), but not isoform 2 (LyP2) or isoform 5. Reactivity toward isoform 6 (PTPN22.6) has not been tested.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PTPN22
      • PTPN8
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~92 kDa observed. 91.7 kDa (isoform 1; Lyp1), 85.1 kDa (isoform 3), 88.6 kDa (isoform 4; Lyp3) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human burkitt's lymphoma Ramos cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected PTPN22 in 10 µg of human burkitt's lymphoma Ramos cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS480 04055977288421