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MABS1899-25UG Anti-Pax-8 Antibody, clone 15C8.1

Anti-PAX-8, clone 15C8.1, Cat. No. MABS1899, is a mouse monoclonal antibody for detection of human Paired box protein Pax-8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Pax-8 Antibody, clone 15C8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Pax8 UniProt Number: Q06710
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MABS1899-25UG
25 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1899-25UG
      DescriptionAnti-Pax-8 Antibody, clone 15C8.1
      Alternate Names
      • Paired box protein Pax-8
      Background InformationPaired box protein Pax-8 (UniProt: Q06710; also known as PAX-8) is encoded by the Pax8 gene (Gene ID: 7849) in human. PAX-8 is a member of the paired box (PAX) family of transcription factors. PAX proteins are important regulators of organogenesis and act as key factors in maintaining pluripotency of stem cells during development. PAX-8 acts as a transcription factor for the thyroid-specific expression of the genes and is exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. PAX-8 is shown to be crucial in determining cell fate during the development of the thyroid, kidney, brain, eyes, and Müllerian system. It also regulates the expression of the Wilms' tumor suppressor gene (WT1). PAX-8 is a shown to be a highly sensitive marker for thyroid, renal, Müllerian, and thymic tumors. However, lung adenocarcinomas, breast and adrenal neoplasms, and the majority of gastrointestinal tumors are reported to be negative for PAX-8. Mutations in Pax8 gene are known to cause congenital hypothyroidism that is characterized by thyroid dysgenesis and in some cases the thyroid gland can be completely absent, ectopically located and/or severely hypoplastic. Five isoforms of PAX-8 have been described that are produced by alternative splicing. (Ref.: Laury, AR et al. (2011). Am J Surg. Pathol. 35(6); 816-826; Xiang, L and Kong, B (2013). Oncol. Lett. 5(3); 735-738).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-PAX-8, clone 15C8.1, Cat. No. MABS1899, is a mouse monoclonal antibody for detection of human Paired box protein Pax-8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Pax-8 in human uterine tube tissue sections.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 129 amino acids from the internal region of human Pax-8.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 15C8.1 detects Pax-8 protein in human ovarian cells. It targets an epitope within 129 amino acids from the internal region.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Pax8
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~50 kDa observed; 48.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in OVCAR3 cell lysate.
      Western Blotting Analysis:  0.5 µg/mL of this antibody detected PAX-8 in 10 µg of OVCAR3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS1899-25UG 04054839543661