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MABE343-AF647 Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate Antibody

This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair
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MABE343-AF647
100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCMAlexaFluor®647Monoclonal Antibody
      Description
      Catalogue NumberMABE343-AF647
      DescriptionAnti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate Antibody
      Background InformationPuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified mouse conjugate in buffer containing PBS, 15 mg/mL BSA, and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenBacteria expressing puromycin-incorporated neosynthesized proteins at multiple molecular weights.
      Clone12D10
      Concentration0.5 mg/mL
      HostMouse
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with cells from all species when pre-incubated with puromycin.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Molecular WeightRefer to Cat. No. MABE343 for observed molecular weight information.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry Analysis untreated and puromycin treated HeLa cells.

      Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected puromycin-incorporated neosynthesized proteins in puromycin treated HeLa cells.

      Alexa Fluor® is a registered trademark of Life Technologies®.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE343-AF647 04053252976308

      Documentation

      Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate Antibody Certificates of Analysis

      TitleLot Number
      Anti-Puromycin, clone 12D10, Alexa -2814777 2814777
      Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate 2991421
      Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate 3012162
      Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate 2952678
      Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate 2922411
      Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate 3062299
      Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate - Q2431444 Q2431444
      Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate - 2906386 2906386
      Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate - 2887487 2887487
      Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate - 3121740 3121740
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies