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MABC679 Anti-RUNX1 Antibody, clone 2B5

Anti-RUNX1 Antibody, clone 2B5 is a highly specific mouse monoclonal antibody & that targets RUNX & has been tested in western blotting.

Anti-RUNX1 Antibody, clone 2B5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: RUNX1, AML1, CBFA2 UniProt Number: Q01196
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MABC679
100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABC679
      DescriptionAnti-RUNX1 Antibody, clone 2B5
      Alternate Names
      • Runt-related transcription factor 1
      • Acute myeloid leukemia 1 protein
      • Core-binding factor subunit alpha-2
      • CBF-alpha-2
        Oncogene AML-1
      • Polyomavirus enhancer-binding protein 2 alpha B subunit
      • PEA2-alpha B
      • PEBP2-alpha B
      • SL3-3 enhancer factor 1 alpha B subunit
      • SL3/AKV core-binding factor alpha B subunit
      Background InformationRunt-related transcription factor 1 (RUNX1), also known as Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2 (CBF-alpha2), Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit (PEA2-alpha B/PEBP2-alpha B), SL3-3 enhancer factor 1 alpha B subunit, and SL3/AKV core-binding factor alpha B subunit is encoded by the human gene named RUNX1/AML1, CBFA2. RUNX1 is a transcription factor that binds to a wide variety of promoters and enhancers belonging to many types of genes from T cell receptor genes to LCK, IL-3 and GM-CSF. Its major normal role is in the development of blood cells and hematopoiesis. It is expressed in most tissues except in normal brain and heart. Levels are highest in the bone marrow, thymus and peripheral lymphocytes. Defects and or translocations involving RUNX1 are the cause for myeloid leukemias as well as lymphoblastic leukemia. Mutations in RUNX1 function are also linked with many breast cancers. Finally, mutations in RUNX1 have recently been shown to be the cause of cancers in epithelial tissues and linked to squamous cell carcinomas. EMD-Millipore’s Anti-RUNX1 mouse monoclonal antibody has been tested in western blot on Jurkat cell lysates and in ELISA using recombinant RUNX1 protein.
      References
      Product Information
      FormatAscites
      Control
      • Jurkat cell lysate
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-RUNX1 Antibody, clone 2B5 is a highly specific mouse monoclonal antibody & that targets RUNX & has been tested in western blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenSynthesized peptide of human RUNX1.
      Clone2B5
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • RUNX1
      • AML1
      • CBFA2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~55 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected RUNX1 in Jurkat cell lysate.

      Optimal working dilutions must be determined by end user.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABC679 04053252917394