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MABF949-SF-25UG Anti-Rabies Virus Antibody, Serum Free Antibody, clone 1112-1

Anti-Rabies Virus, Serum Free, clone 1112-1, Cat. No. MABF949-SF, is a mouse monoclonal antibody that detects Glycoprotein of rabies virus., strain ERA and has been tested for use in ELISA, Flow Cytometry, and Neutralizing applications.

Research Category: Inflammation & Immunology UniProt Number: P03524
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MABF949-SF-25UG
25 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VirusELISA, FC, NEUTMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF949-SF-25UG
      DescriptionAnti-Rabies Virus Antibody, Serum Free Antibody, clone 1112-1
      Alternate Names
      • Glycoprotein
      • Glycoprotein Rabies virus
      Background InformationGlycoprotein (UniProt: P03524) is encoded by the G gene in Rabies virus, strain ERA. Glycoprotein is a single-pass type I membrane protein that belongs to the lyssavirus glycoprotein family. It attached the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. The muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) are reported to bind glycoprotein and thereby facilitate rabies virus entry into cells. Glycoprotein is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. Its structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524). Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. Glycoprotein is glycosylated and palmitoylated by host and glycosylation is considered to be crucial for glycoprotein export at the cell surface. Clone 1112-1 is a serum-free version and is shown to neutralize Rabies Virus, CVS-11 strain. (Ref.: Dietzschold, B et al. (1992). Proc. Natl. Acad. Sci. USA 89 (15); 7252-7256; Wiktor, TJ and Koprowski, H (1980). J. Exp. Med. 152: 99-112; Marissen, WE et al (2005). J. Virol. 79(8): 4672-4678).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Applications
      ApplicationAnti-Rabies Virus, Serum Free, clone 1112-1, Cat. No. MABF949-SF, is a mouse monoclonal antibody that detects Glycoprotein of rabies virus., strain ERA and has been tested for use in ELISA, Flow Cytometry, and Neutralizing applications.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Neutralizing
      Application NotesNeutralizing Analysis: A representative lot neutralized Rabies Virus, Serum Free CVS-11 strain in Neutralizing applications (Bunschoten, H., et. al. (1989). J Gen. Virol. 70 ( Pt 2):291-8; Dietzschold, B., et. al. (1992). Proc Natl Acad Sci USA. 89(15):7252-6; Muller, T., et. al. (2009). PLoS Negl Trop Dis. 3(11):e542; Schumacher, C.L., et. al. (1989). J Clin Invest. 84(3):971-5).

      ELISA Analysis: A representative lot detected Rabies Virus, Serum Free in ELISA applications (Morgeaux, S., et. al. (2017). Vaccine. 35(6):966-971).
      Biological Information
      ImmunogenProtein G of Evelyn Rokitniki Abelseth(ERA) SAD derived RABV strain).
      Clone1112-1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1112-1 is a mouse monoclonal antibody that detects detects glycoprotein of strain ERA rabbies virus.
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteRabies Virus.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight58.66 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in L929 cells expressing Rabies virus glycoprotein.

      Flow Cytometry Analysis: 2 µg of this antibody detected Rabies Virus, Serum Free in L929 cells expressing Rabies virus glycoprotein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABF949-SF-25UG 04054839519406

      Documentation