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MABF3038-100UG Anti-Rhinovirus Capsid VP1 Antibody, clone HRV-18003

Anti-Rhinovirus Capsid VP1, clone HRV-18003, Cat. No. MABF3038, is a mouse monoclonal antibody that detects Rhinovirus capsid VP1 and is tested for use in ELISA, Functional Assay, and Western Blotting.

MABF3038-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3038-100UG
      DescriptionAnti-Rhinovirus Capsid VP1 Antibody, clone HRV-18003
      Alternate Names
      • HRV-capsid VP1
      Background InformationRhinoviruses (RV) belong to the Picornavirus family and are known as a leading cause of respiratory infections. Over 160 different types of rhinoviruses have been identified and based on their genetic diversity and phylogenetic sequence analysis they are grouped as rhinovirus A, B, and C. Three different membrane glycoproteins are known to serve as receptors for these viruses. ICAM-1 is used by a majority of A group and all B group rhinoviruses, a small number of group A viruses use the low-density lipoprotein receptor (LDLR), and the group C of rhinoviruses use cadherin-related family member 3 receptor (CDKR3). Canyons on the surface of the virus are known to provide an attachment site for receptors on the surface of susceptible target cells. Viral infectivity is neutralized when host immunoglobulin G binds to the surface of the virus, thereby blocking interaction between the host cell receptor and the receptor binding site located at the base of the canyon. The RV is composed of single-stranded RNA within a capsid with icosahedral symmetry. The viral capsid of human RV is comprised of four viral proteins: VP1, VP2, VP3, and VP4. The remaining viral proteins are responsible for viral replication and subsequent assembly. Capsid protein VP1 forms an icosahedral capsid of pseudo-T=3 symmetry with capsid proteins VP2 and VP3. VP1 interacts with host cell receptor to provide virion attachment to target host cells. This attachment induces virion internalization. VP1 is the most exposed surface protein and plays a critical role in viral antigenicity and induction of neutralizing antibodies. Clone HRV-18003 binds to a conserve epitope within VP1 and displays cross reactivity with multiple types of rhinoviruses, including A1A, A1B, A15, and A49. It exhibits strong antibody dependent cellular phagocytosis (ADCP) against A1A and A16 viruses. (Ref.: Behzadi, MA., et al. (2020). Sci. Rep. 10(1):9750; Basnet, S., et al. (2019). Chest. ;155(5):1018-1025).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Rhinovirus Capsid VP1, clone HRV-18003, Cat. No. MABF3038, is a mouse monoclonal antibody that detects Rhinovirus capsid VP1 and is tested for use in ELISA, Functional Assay, and Western Blotting.
      Key Applications
      • Western Blotting
      • Affects Function
      • ELISA
      Application NotesTested Applications

      Functional Assay A representative lot of this antibody exhibited strong activity in in vitro antibody dependent cellular phagocytosis (ADCP) assay. (Behzadi, M.A., et al. (2020). Sci Rep.;10(1):9750).

      Western Blotting Analysis: A representative lot detected HRV-18003 recognizes VP1 in Western Blotting applications (Behzadi, M.A., et al. (2020). Sci Rep.;10(1):9750).

      ELISA Analysis: A representative lot detected HRV-18003 recognizes VP1 in ELISA applications (Behzadi, M.A., et al. (2020). Sci Rep.;10(1):9750).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenDNA encoding P1-2A and 3C of different strains of Rhinoviruses in pCAGGS plasmids.
      EpitopeN-terminal half
      CloneHRV-18003
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone HRV-18003 is a mouse monoclonal antibody that detects Rhinovirus. It targets an epitope within 8 amino acids from the N-terminal half.
      IsotypeIgG2aκ
      Species Reactivity
      • Virus
      Species Reactivity NoteRhinovirus.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~32 kDa observed for Capsid VP1 protein. 242.82 kDa calculated for the whole Genome polyprotein. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant Rhinovirus Capsid VP1 protein.

      Western Blotting Analysis: A 1:125 dilution of this antibody detected recombinant Rhinovirus Capsid VP1 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABF3038-100UG 04065270647184

      Documentation

      Anti-Rhinovirus Capsid VP1 Antibody, clone HRV-18003 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Rhinovirus Capsid VP1 Antibody, clone HRV-18003 Certificates of Analysis

      TitleLot Number
      Anti-Rhinovirus Capsid VP1, clone HRV-18003 - 4172551 4172551
      Anti-Rhinovirus Capsid VP1, clone HRV-18003 - 4201240 4201240
      Anti-Rhinovirus Capsid VP1, clone HRV-18003 - 4203330 4203330
      Anti-Rhinovirus Capsid VP1, clone HRV-18003 - 4208460 4208460
      Anti-Rhinovirus Capsid VP1, clone HRV-18003 - 4219070 4219070
      Anti-Rhinovirus Capsid VP1, clone HRV-18003 - 4222040 4222040
      Anti-Rhinovirus Capsid VP1, clone HRV-18003 - 4222907 4222907
      Anti-Rhinovirus Capsid VP1, clone HRV-18003 - 4228984 4228984
      Anti-Rhinovirus Capsid VP1, clone HRV-18003 - 4229980 4229980
      Anti-Rhinovirus Capsid VP1, clone HRV-18003 - Q4075626 Q4075626