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ABE1311 Anti-SETD5

Anti-SETD5, Cat. No. ABE1311, is a highly specific rabbit polyclonal antibody that targets SET domain-containing protein 5 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-SETD5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: SETD5, KIAA1757 UniProt Number: Q9C0A6
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ABE1311
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IH(P), WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1311
      DescriptionAnti-SETD5
      Alternate Names
      • SET domain-containing protein 5
      Background InformationSET domain-containing protein 5 (UniProt: Q9C0A6; also known as Setd5) is encoded by the SETD5 (also known as KIAA1757) gene (Gene ID: 55209) in human. Setd5 protein is shown to be essential for mammalian development and is required for regulating germ cell-associated genes in embryonic stem cells. It interacts with Tbl1xr1 and Ctr9, which are components of 2 different transcriptional regulatory complexes (NcoR1 corepressor complex and Paf1 complex) in murine embryonic stem cells. Setd5-deficient embryonic stem cells display impaired cellular proliferation, increased apoptosis, defective cell cycle progression, a diminished ability to differentiate into cardiomyocytes, and greatly perturbed gene expression. Setd5-deficient mouse embryos exhibit severe defects in neural tube formation, somitogenesis and cardiac development, have aberrant vasculogenesis in embryos, yolk sacs, and placentas. They die between embryonic day 10.5 and 11.5. Three isoforms of Setd5 have been reported that are produced by alternative splicing. Its SET domain is localized to amino acids 269-390. Mutation in SETD5 gene has been linked to intellectual disability. (Ref.: Yu, SE., et al. (2017). Cell. Biochem. Funct. 35(5); 247-253; Osipovich, AB., et al. (2016). Development 143(24); 4595-4607).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SETD5, Cat. No. ABE1311, is a highly specific rabbit polyclonal antibody that targets SET domain-containing protein 5 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SETD5 in human cerebral cortex, human pancreas, and human testis tissue sections.

      Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SETD5 in U2OS cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human SET domain-containing protein 5.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects SET domain containing protein 5 in human cells. It targets an epitope within 19 amino acids from the N-terminal region.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rat and Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SETD5
      • KIAA1757
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~150 kDa observed; 158.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected SETD5 in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABE1311 04054839415098