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07-645-25UG Anti-Sp1 (rabbit polyclonal)

Use Anti-Sp1 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, EMSA, IF, IP, WB to detect Sp1 also known as Sp1 transcription factor, specificity protein 1.

Anti-Sp1 (rabbit polyclonal) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: TSFP1 UniProt Number: P08047
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07-645-25UG
25 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, REMSA, IF, IP, WB, ChIPRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-645-25UG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Sp1 (rabbit polyclonal)
      Alternate Names
      • Sp1 transcription factor
      • specificity protein 1
      Background InformationSp1 is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 95 kDA. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.
      References
      Product Information
      FormatPurified
      Control
      • HeLa nuclear extract.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-Sp1 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, EMSA, IF, IP, WB to detect Sp1 also known as Sp1 transcription factor, specificity protein 1.
      Key Applications
      • Electrophoretic Mobility Shift Assay
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunoprecipitation:
      4 µg of this lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
      NOTE: A previous lot successfully immunoprecipitated the α-1 subunit using the Catch and Release v2.0 Reversible Immunoprecipitation System (Catalog # 17-500). See manual for more information and protocol: www.millipore.com

      Chromatin Immunoprecipitation:
      Reported by an independent laboratory.

      Immunofluorescence:
      Reported by an independent laboratory.

      Electrophoretic Mobility Shift Assay (EMSA):
      1 µL of a previous lot of this antibody has been reported by an independent laboratory to supershift DNA complexes containing recombinant Sp1 and endogenous Sp1 from HeLa cells in EMSA.
      Biological Information
      ImmunogenFull length human Sp1 protein.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityRecognizes Sp1, Mr 105 kDa and 95 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman and mouse. Expected to cross react with rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TSFP1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08047 # Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.
      SIZE: 785 amino acids; 80693 Da
      SUBUNIT: Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: O-glycosylated; contains N-acetylglucosamine side chains.
      SIMILARITY: SwissProt: P08047 ## Belongs to the Sp1 C2H2-type zinc-finger protein family. & Contains 3 C2H2-type zinc fingers.
      Molecular WeightVaries
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected SP1 on 10 μg of HeLa lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      07-645-25UG 04054839337390