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07-1527 Anti-TFIIF-alpha Antibody

Use Anti-TFIIF-alpha Antibody (rabbit polyclonal antibody) validated in WB, IP to detect TFIIF-alpha also known as Transcription initiation factor IIF subunit alpha, Transcription initiation factor RAP74.

Anti-TFIIF-alpha Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: GTF2F1, RAP74 UniProt Number: P35269
07-1527
100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, B, Po, ChpWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1527
      DescriptionAnti-TFIIF-alpha Antibody
      Alternate Names
      • General transcription factor IIF subunit 1
      • General transcription factor IIF 74 kDa subunit
      • Transcription initiation factor IIF subunit alpha
      • TFIIF-alpha
      • Transcription initiation factor RAP74
      Background InformationTFIIF, a general transcription initiation factor, is a heterodimer composed of 74 kDa, GTF2F1 (Rap74), and a 30 kDa (GTF2F2) subunits. TFIIF is involved in transcription initiation and elongation, and the CTD of GTF2F1 binds to the RNA polymerase II enzyme, the general transcription factor TFIIB, and a CTD phosphate, FCP1. Studies point to TFIIF involvement in both early and late transcriptional stages. Data also suggest that GTF2F1 is involved in the in the recycling of the RNA Polymerase II via FCP1's CTD-specific phosphatase activity.
      References
      Product Information
      FormatAffinity Purified
      Control
      • K562 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-TFIIF-alpha Antibody (rabbit polyclonal antibody) validated in WB, IP to detect TFIIF-alpha also known as Transcription initiation factor IIF subunit alpha, Transcription initiation factor RAP74.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated TFIIF-alpha from K562 RIPA lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human TFIIF-alpha at the C-terminus.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes GTF2F1 at the C-terminus.
      Species Reactivity
      • Human
      • Bovine
      • Pig
      • Chimpanzee
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Bovine, Porcine, Orangutan, and Chimpanzee based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GTF2F1
      • RAP74
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

      SUBUNIT STRUCTURE: Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2 and CTDP1. Interacts with TAF6/TAFII80.

      SUBCELLULAR LOCATION: Nucleus.

      INDUCTION: Up-regulated in response to enterovirus 71 (EV71) infection.

      PTM: Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases.

      SEQUENCE SIMILARITIES: Belongs to the TFIIF alpha subunit family.

      CAUTION: Was reported (Ref.9) to have a protein kinase activity and to autophosphorylates on Ser-385 and Thr-389.
      Molecular Weight~74 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in K562 cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected TFIIF-alpha on 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      07-1527 04053252421136