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610205 Anti-Tie2 Mouse mAb (Ab33)

This Anti-Tie2 Mouse mAb (Ab33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tie2.

Anti-Tie2 Mouse mAb (Ab33) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Angiopoietin Receptor, Anti-Tek

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610205
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, Porcine, RMMonoclonal Antibody

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
610205-50UG
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      		 Plastic ampoule 50 μg
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      Description
      OverviewRecognizes the ~140-45 kDa Tie2 protein.
      Catalogue Number610205
      Brand Family Calbiochem®
      SynonymsAnti-Angiopoietin Receptor, Anti-Tek
      References
      ReferencesLin, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 8829.
      Wong, A.L., et al. 1997. Circ. Res. 81, 567.
      Peters, K.G., et al. 1998. Br. J. Cancer 77, 51.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Not Paraffin Sections
      Application NotesFrozen Sections (see comments)
      Immunoblotting (0.2-1 µg/ml, chemiluminescence)
      Immunoprecipitation (see comments)
      Paraffin Sections (not recommended)
      Application CommentsFor frozen sections, tissues were treated with Triton®-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag® sequence
      ImmunogenHuman
      CloneAb33
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Porcine
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      610205-50UG 04055977264197

      Documentation

      Anti-Tie2 Mouse mAb (Ab33) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tie2 Mouse mAb (Ab33) Certificates of Analysis

      TitleLot Number
      610205

      References

      Reference overview
      Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 8829.
      Wong, A.L., et al. 1997. Circ. Res. 81, 567.
      Peters, K.G., et al. 1998. Br. J. Cancer 77, 51.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-November-2017 JSW
      SynonymsAnti-Angiopoietin Receptor, Anti-Tek
      ApplicationFrozen Sections (see comments)
      Immunoblotting (0.2-1 µg/ml, chemiluminescence)
      Immunoprecipitation (see comments)
      Paraffin Sections (not recommended)
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~140-145 kDa Tie2 protein.
      BackgroundAngiopoietin-1 and its naturally occurring inhibitor, angiopoietin-2, exert their effects by inducing autophosphorylation of their signaling receptor Tie2. Phosphorylation of the receptor occurs during vasculogenesis and is required for its activity in regulating endothelial cell proliferation, differentiation and guiding the proper patterning of endothelial cells during blood vessel formation. Tie-2 is a transmembrane receptor-tyrosine kinase that is expressed by endothelial cells and primitive hematopoietic tissues. Tie2/Tek has been shown to be critical for endothelial cell survival, which is evidenced in part by the constitutive phosphorylation that is observed in quiescent endothelium. More recently a multifunction docking site has been identified on the receptor (Tyr1100) that serves as a binding site for other partners including Grb2, Grb7, and p85 and acting via an SH2 domain interaction.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag® sequence
      CloneAb33
      IsotypeIgG₁
      Specieshuman, mouse, porcine, rat
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor frozen sections, tissues were treated with Triton®-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesLin, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 8829.
      Wong, A.L., et al. 1997. Circ. Res. 81, 567.
      Peters, K.G., et al. 1998. Br. J. Cancer 77, 51.